Sigma-Aldrich recently discontinued the ELISA kit for determination of vitamin D in plasma/serum.
Dear Yogita, every Vitamin D ELISA validated for serum samples should do. Alternatively, you might check with a hospital that performs vitamin D assays in their clinical chemistry lab, maybe they want to cooperate.
I would use any that has a VD-DBP dissociation step included, so you could avoid doing it with organic solvents (acetone). ENZO has a product like this, relatively expensive, though. If you don't mind solvents, CAYMAN is a good, less expensive choice.
I wouldn't care about acetone etc. unless it's increasing the costs in a unreasonable way. Everything in life is chemistry and acetone is really harmless, unless you drink it. Would you?
Regardless the solvents, dissociation step is time and resource consuming: you have to add acetone to the samples, centrifuge, pass the supernatant to glass tubes, let the acetone air dry and reconstitute the samples before the assay (at least for CAYMAN's kit). This may be troublesome when analyzing hundreds of samples.
You should take all this (besides money) in consideration so to make a wise choice.
Good luck
Thank you for all answers.
Is Immunodiagnostics and IDS kit good for Vitamin D? Anyone used it?
Among the many kits on the market that are reliable and well calibrated, I would prefer the vitamin D total kit of Roche. And indeed seek a cooperation with a medical lab in your neigbourhood, I am sure they will run your samples at a fair price.
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