Which wavelength is suitable for plant regeneration from explant of orchidaceae?

07 July 2013 1 7K Report

Which wavelength will produce responses in plant tissue culture?

Here is a useful system simulating natural sunlight.

https://www.researchgate.net/publication/255626668_Can_we_use_Elevated_Carbon_Dioxide_to_Increase_Productivity_in_the_Orchid_Industry?ev=prf_pub

Article Can we use Elevated Carbon Dioxide to Increase Productivity ...

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Protein concentration in EMSA reaction?

Can anyone tell me a rough protein final concentration for a good start? I am using 20fmol biotinylated DNA in 20ul reaction volume. What can be good amount of DNA binding protein to start with?

11 December 2016 5,624 3 View

Sequences of pGWB3 vector with GUS reporter?

Can any one provide me the complete sequence of pGWB3 plant expression vector?

04 May 2015 10,144 4 View

Why does reaction volume decreases after restriction digestion of plasmid?

I have noticed that my reaction volume has been decreased by 2-3 ul after the restriction digestion of plasmids with different restriction enzymes at 37 °C. I am sure that there is no or very...

07 August 2014 8,367 4 View

How to design Real time PCR primers?

I want to design real time PCR primers for my gene of interest which is 1Kb in size and with one intron. What are the important parameters that I should keep in mind while designing primers? Is...

06 July 2014 3,516 8 View

What kind of expression and purification system can be used?

My protein of interest (human) contains glycosylation and having 34 kDa molecular weight. For crystallization purpose.

10 November 2013 2,976 1 View

How can I activate my oncogene of interest in plants?

I want to silence an oncogene (which is transfected) for a period of time and activate it. How can I do that? Could you suggest some papers for the above case,if any? This is a general case.

10 November 2013 3,791 1 View

Which ribonucleic acid sequence gives better results in RNA interference mediated gene silencing in plants?

I would like to know which sequence (shRNA, siRNA etc) is appropriate for gene silencing in plant species? What are the factors that should be considered for selection of RNA sequence?

10 November 2013 8,733 2 View

Does anybody know the plant hormones which are secreted at the time of fruit formation in plants?

I am specifically interested in plant hormones which are either only secreted at the time of fruit formation or which are down regulated at that time. No need of specificity, can be any plant.

10 November 2013 10,032 12 View

What are the different RNA interference methods?

I would like to know more on their advantages, disadvantage and their use in gene expression analysis.

10 November 2013 7,372 0 View

What are the best methods to evaluate and compare the gene expression in plants?

I want to check the gene expressions of two family of plants. What are the methods that I can use? I also want to know the limitations. A discussion on this topic is expected.

10 November 2013 2,508 3 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View