How can I get the specific RNA expression of an alternative transcript of a gene of interest?

10 November 2013 3,280 7 View

What tools do you use for illustrating your articles?

Each time I write an article, I have to struggle with the problem of the illustrations. I can often see pretty nice figures in articles, more especially in reviews, which makes me think that my...

05 June 2013 376 21 View

How far can one go in functional annotation of variants with the Zebrafish model?

I have seen a pretty interesting presentation by Dr. Nicholas Katsanis at the ESHG meeting who presented his work on functional annotation of variants found by exome sequencing. From what I knew...

05 June 2013 7,466 11 View

What is the best algorithm for detection of insertions/duplications in NGS data?

I have been working on data analyzed by Samtools or GATK, and in both cases indels were badly detected. More especially, duplications or small insertions were missed by the two tools. I had...

11 December 2012 3,580 10 View

What would you use to explore the tissue distribution of an unknown gene?

I'm looking for an alternative to hybridization assays of northern blots. I used this technique before, but it's outdated now. I assume that there are plenty of ways to examine the expression of a...

10 November 2012 9,650 6 View

Is the title of Doctor restricted to physicians?

09 October 2012 2,271 31 View

If you had to conduct a GWAS today, what strategy would you follow?

SNP array? SNP array + low coverage genome sequencing? CNVs?

09 October 2012 2,473 15 View

Does anyone have any experience in determining allele frequency in pooled DNA by targeted high-throughput sequencing?

I'm looking for a cost-effective means for validation of potential associations in a GWAS as an alternative to individual genotyping, at least in a first time.

09 October 2012 1,680 2 View

Do you know any user-friendly tools for parsing and/or handling variant call files?

I'm looking for tools working under windows environment with java that could be used as atlernatives to vcftools or even GATK.

09 October 2012 4,027 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View