I would like to express a gene of interest, but I haven't a precise idea of the vector I should use. If you have any experience in this field, I would be pleased to read your posts.
I found a mutation in an alternative transcript of a human gene, which is not the main transcript – it is associated with a CDS, though. Correct me if I'm wrong, but usually RNA expression...
10 November 2013 3,406 7 View
Each time I write an article, I have to struggle with the problem of the illustrations. I can often see pretty nice figures in articles, more especially in reviews, which makes me think that my...
05 June 2013 518 21 View
I have seen a pretty interesting presentation by Dr. Nicholas Katsanis at the ESHG meeting who presented his work on functional annotation of variants found by exome sequencing. From what I knew...
05 June 2013 7,636 11 View
I have been working on data analyzed by Samtools or GATK, and in both cases indels were badly detected. More especially, duplications or small insertions were missed by the two tools. I had...
11 December 2012 3,702 10 View
I'm looking for an alternative to hybridization assays of northern blots. I used this technique before, but it's outdated now. I assume that there are plenty of ways to examine the expression of a...
10 November 2012 9,771 6 View
In France, people associate the name of Doctor with physicians. If you ask people in the street, Doctor is synonymous with Medical Doctor. It’s more disturbing to note that even in hospitals, only...
09 October 2012 2,653 31 View
SNP array? SNP array + low coverage genome sequencing? CNVs?
09 October 2012 2,590 15 View
I'm looking for tools working under windows environment with java that could be used as atlernatives to vcftools or even GATK.
09 October 2012 4,145 3 View
I'm looking for a cost-effective means for validation of potential associations in a GWAS as an alternative to individual genotyping, at least in a first time.
09 October 2012 1,807 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View