How can I get the specific RNA expression of an alternative transcript of a gene of interest?

10 November 2013 3,280 7 View

What tools do you use for illustrating your articles?

Each time I write an article, I have to struggle with the problem of the illustrations. I can often see pretty nice figures in articles, more especially in reviews, which makes me think that my...

05 June 2013 376 21 View

Which vector would you use for cDNA expression in human fibroblasts?

I would like to express a gene of interest, but I haven't a precise idea of the vector I should use. If you have any experience in this field, I would be pleased to read your posts.

05 June 2013 2,307 0 View

How far can one go in functional annotation of variants with the Zebrafish model?

I have seen a pretty interesting presentation by Dr. Nicholas Katsanis at the ESHG meeting who presented his work on functional annotation of variants found by exome sequencing. From what I knew...

05 June 2013 7,466 11 View

What is the best algorithm for detection of insertions/duplications in NGS data?

I have been working on data analyzed by Samtools or GATK, and in both cases indels were badly detected. More especially, duplications or small insertions were missed by the two tools. I had...

11 December 2012 3,580 10 View

What would you use to explore the tissue distribution of an unknown gene?

I'm looking for an alternative to hybridization assays of northern blots. I used this technique before, but it's outdated now. I assume that there are plenty of ways to examine the expression of a...

10 November 2012 9,650 6 View

Is the title of Doctor restricted to physicians?

09 October 2012 2,271 31 View

If you had to conduct a GWAS today, what strategy would you follow?

SNP array? SNP array + low coverage genome sequencing? CNVs?

09 October 2012 2,473 15 View

Does anyone have any experience in determining allele frequency in pooled DNA by targeted high-throughput sequencing?

I'm looking for a cost-effective means for validation of potential associations in a GWAS as an alternative to individual genotyping, at least in a first time.

09 October 2012 1,680 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View