Sébastien Küry

10 Questions 74 Answers 0 Followers

Questions related from Sébastien Küry

How can I get the specific RNA expression of an alternative transcript of a gene of interest?

I found a mutation in an alternative transcript of a human gene, which is not the main transcript – it is associated with a CDS, though. Correct me if I'm wrong, but usually RNA expression...

11 November 2013 3,353 7 View

What tools do you use for illustrating your articles?

Each time I write an article, I have to struggle with the problem of the illustrations. I can often see pretty nice figures in articles, more especially in reviews, which makes me think that my...

06 June 2013 452 21 View

How far can one go in functional annotation of variants with the Zebrafish model?

I have seen a pretty interesting presentation by Dr. Nicholas Katsanis at the ESHG meeting who presented his work on functional annotation of variants found by exome sequencing. From what I knew...

06 June 2013 7,550 11 View

Which vector would you use for cDNA expression in human fibroblasts?

I would like to express a gene of interest, but I haven't a precise idea of the vector I should use. If you have any experience in this field, I would be pleased to read your posts.

06 June 2013 2,393 0 View

What is the best algorithm for detection of insertions/duplications in NGS data?

I have been working on data analyzed by Samtools or GATK, and in both cases indels were badly detected. More especially, duplications or small insertions were missed by the two tools. I had...

12 December 2012 3,644 10 View

What would you use to explore the tissue distribution of an unknown gene?

I'm looking for an alternative to hybridization assays of northern blots. I used this technique before, but it's outdated now. I assume that there are plenty of ways to examine the expression of a...

11 November 2012 9,714 6 View

Is the title of Doctor restricted to physicians?

In France, people associate the name of Doctor with physicians. If you ask people in the street, Doctor is synonymous with Medical Doctor. It’s more disturbing to note that even in hospitals, only...

10 October 2012 2,425 31 View

Do you know any user-friendly tools for parsing and/or handling variant call files?

I'm looking for tools working under windows environment with java that could be used as atlernatives to vcftools or even GATK.

10 October 2012 4,088 3 View

If you had to conduct a GWAS today, what strategy would you follow?

SNP array? SNP array + low coverage genome sequencing? CNVs?

10 October 2012 2,536 15 View

Does anyone have any experience in determining allele frequency in pooled DNA by targeted high-throughput sequencing?

I'm looking for a cost-effective means for validation of potential associations in a GWAS as an alternative to individual genotyping, at least in a first time.

10 October 2012 1,748 2 View