Which type/grade of CHAPS should be used for Protein resuspension (for trizol protein)? Is there any alternative for protein resuspension?

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Can anyone provide me with working protocol for staining Neurofibrillary tangles?

I have come across Gallyas Silver stain and Bielschowsky's Silver Staining Protocol for Nerve Fibers, Axons, Neurofibrillary Tangles. Has anyone tried this methods of staining ? Can anyone provide...

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Is it necessary to invert the Western image before measuring integrated density in Image J?

Before doing Integrated density measurement in image J software is iit necessary to invert the Western blot image (from black protein band and white background to white protein band and black...

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Can anyone provide me with working protocol for ECL solution preparation for Western Blotting?

Since ECL is provided from many companies viz Thermo, biorad etc. which is bit costly. Can anyone provide with me with the working laboratory protocol for ECL preparation for Western...

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Have anyone tried IL-1 alpha antibody for Western blotting by using mouse brain tissue samples?

Have anyone tried IL-1alpha antibody for western blotting using mouse tissue samples? I have used abcam Il-1 alpha. As per the information sheet two bands were reported (one at 17 kDa and other at...

11 December 2015 2,486 5 View

How can I determine Homocysteine levels in mouse serum/plasma?

As per I searched, there are two methods for homocysteine levels determination. HPLC and a kit method. Which is the best and convinient method ? If HPLC, can anyone share the working protocol? and...

11 December 2015 960 2 View

Pellet is forming again and again in the supernatant after using HEPES lysis buffer for protein isolation! Why it is so?

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How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

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Can I use a HisTRAP column for affinity chromatography?

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Why does my protein refolded to beta sheet during thermal denaturation analysis?

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What is the best blank for nanodrop if I want to read a recombinant protein concentration?

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Transfection in HEK293T cells?

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Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

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How to retain the a GFP tagged gene expression in stable cell line?

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What is the relationship between protein structure and N or C terminal tagging choosing?

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Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

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Problems with ion exchange chromatography?

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Hung King Tiong

Tris-buffered urea

The information can be found at:

http://www.ncbi.nlm.nih.gov/pubmed/25578509

Comparison of five methods for direct extraction of surface proteins from Listeria monocytogenes for proteomic analysis by orbitrap mass spectrometry.

Pranav Joshi

Thank you

wish u success

Thank you sir