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Questions related from Pranav Joshi
I have come across Gallyas Silver stain and Bielschowsky's Silver Staining Protocol for Nerve Fibers, Axons, Neurofibrillary Tangles. Has anyone tried this methods of staining ? Can anyone provide...
05 May 2016 4,157 4 View
Before doing Integrated density measurement in image J software is iit necessary to invert the Western blot image (from black protein band and white background to white protein band and black...
04 April 2016 3,780 5 View
Since ECL is provided from many companies viz Thermo, biorad etc. which is bit costly. Can anyone provide with me with the working laboratory protocol for ECL preparation for Western...
02 February 2016 1,579 5 View
Have anyone tried IL-1alpha antibody for western blotting using mouse tissue samples? I have used abcam Il-1 alpha. As per the information sheet two bands were reported (one at 17 kDa and other at...
12 December 2015 2,455 5 View
As per I searched, there are two methods for homocysteine levels determination. HPLC and a kit method. Which is the best and convinient method ? If HPLC, can anyone share the working protocol? and...
12 December 2015 922 2 View
I once tried HEPES lysis buffer for tissue lysis to obtain protein. After homogenization and sonication , i centrifused and collected the supernatant for protein as per the protocol. but later on...
04 April 2015 6,133 5 View
Which type/grade of CHAPS should be used for Protein resuspension (for trizol protein)? Is there any alternative for protein resuspension?
02 February 2015 4,790 4 View
