Can anyone provide me with working protocol for staining Neurofibrillary tangles?

04 May 2016 4,100 4 View

Is it necessary to invert the Western image before measuring integrated density in Image J?

03 April 2016 3,716 5 View

Can anyone provide me with working protocol for ECL solution preparation for Western Blotting?

Since ECL is provided from many companies viz Thermo, biorad etc. which is bit costly. Can anyone provide with me with the working laboratory protocol for ECL preparation for Western...

01 February 2016 1,533 5 View

Have anyone tried IL-1 alpha antibody for Western blotting by using mouse brain tissue samples?

Have anyone tried IL-1alpha antibody for western blotting using mouse tissue samples? I have used abcam Il-1 alpha. As per the information sheet two bands were reported (one at 17 kDa and other at...

11 December 2015 2,398 5 View

Pellet is forming again and again in the supernatant after using HEPES lysis buffer for protein isolation! Why it is so?

I once tried HEPES lysis buffer for tissue lysis to obtain protein. After homogenization and sonication , i centrifused and collected the supernatant for protein as per the protocol. but later on...

03 April 2015 6,084 5 View

Which type/grade of CHAPS should be used for Protein resuspension (for trizol protein)? Is there any alternative for protein resuspension?

Which type/grade of CHAPS should be used for Protein resuspension (for trizol protein)? Is there any alternative for protein resuspension?

01 February 2015 4,741 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View