For secondary metabolite estimation, the crude extract of A . flavus was obtained by extracting the culture filtrate with ethyl acetate. What must be the suitable solvent for suspending the extract for further qualitative estimation such as Total Phenolic and Flavonoid content? I tried hexane and methanol. But the extract was insoluble in methanol while dissolving in hexane wasn't beneficial as hexane is insoluble in water, thereby hindering further analytic procedures.
50 % aqueous methanol (if you have phenolics there mostly!), 20 % Tween 20 or DMSO (10% or higher) would also help, in addition to warming the extract/ sonication during dissolution. Use these as appropriate blanks during phenolic estimation and should be fine.
Thanks a tonne Mr. Misra!
I had tried dissolving it in 100% MeOH but the solution turned milky white, almost opaque. Will try with 50% now..
Secondary metabolite in crude extract of A . flavus was obtained by extracting the culture filtrate with ethyl acetate. You can use butanol and methanol, or you can use DMSO 5% for suspending the crude extract of A. flavus.
If you want to perform analysis then go for methanol and chloroform (1:1). For pharmacological screening go for suspending your extract in water using tween 80 as surfactant.
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