Various researchers are using different mg/ml like 1 or 5 but which one is the most appropriate
The ration at 5 is better, but the most appropriate one maybe obtained according to the materials you wanti to calcify.
The ratio at 5 is better, but the most appropriate one maybe obtained according to the materials you wanti to calcify.
The more – the better because during the test, precipitation of calcium phosphate occurs, therefore, concentration of these ions in SBF solution decreases. The more amount of SBF is used – the less noticeable this effect is.
I use ISO/FDIS 23317 the Implants for surgery — In vitro evaluation for apatite-forming ability of implant materials.
For calculate the ratio I use the following equation:
Vs = Sa/10
Vs is the volume of the SBF in cubic millimetres
Sa is the apparent surface area of the specimen in square millimetres
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