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Questions related from Kartikeya Rajpal
I need to study the cell morphology of a fungal hyphae, specifically cell wall and cell membrane. Which technique is better - confocal microscopy or Total internal reflection fluorescence...
27 October 2015 3,410 4 View
Having done secondary metabolite profiling of a particular fungus, the next step is to classify the metabolites into various classes such as polyketides, NRPs, terpenoids, etc. How should one go...
05 July 2015 2,038 3 View
How does NaCl affects the metabolism of a fungi? NaCl up to a certain concentration promotes fungal growth whereas at higher concentration inhibits it. Can anybody elaborate on the mechanism...
07 March 2015 3,681 5 View
Is there a way of visualizing the intracellular localization of fungal secondary metabolite?
20 February 2015 8,322 7 View
I have tried 2 protocols for protease assay but both have given no results. Can someone please suggest a tried and tested protocol for determination of fungal protease activity?
21 January 2015 9,208 2 View
I have a fungal isolate of which I need to do the secondary metabolite profiling. Which of the two analytical techniques should I go for - HPLC or LC-MS? Should I directly go for the LC-MS of the...
30 December 2014 9,179 3 View
For secondary metabolite estimation, the crude extract of A . flavus was obtained by extracting the culture filtrate with ethyl acetate. What must be the suitable solvent for suspending the...
07 December 2014 2,177 4 View
