Kartikeya Rajpal

6 Questions 16 Answers 0 Followers

Questions related from Kartikeya Rajpal

Which fluorescence microscopy should I use for imaging cell wall and cell membrane of Aspergillus?

I need to study the cell morphology of a fungal hyphae, specifically cell wall and cell membrane. Which technique is better - confocal microscopy or Total internal reflection fluorescence...

27 October 2015 3,375 4 View

What's the effect of NaCl on fungal metabolism?

How does NaCl affects the metabolism of a fungi? NaCl up to a certain concentration promotes fungal growth whereas at higher concentration inhibits it. Can anybody elaborate on the mechanism...

07 March 2015 3,640 5 View

Is there a way of visualizing the intracellular localization of fungal secondary metabolite?

20 February 2015 8,279 7 View

Can someone please suggest a protocol for fungal protease assay?

I have tried 2 protocols for protease assay but both have given no results. Can someone please suggest a tried and tested protocol for determination of fungal protease activity?

21 January 2015 9,168 2 View

What's the best analytical technique for secondary metabolite profiling of a fungus?

I have a fungal isolate of which I need to do the secondary metabolite profiling. Which of the two analytical techniques should I go for - HPLC or LC-MS? Should I directly go for the LC-MS of the...

30 December 2014 9,138 3 View

Which solvents are suitable for suspending the crude extract of A. flavus, obtained by ethyl acetate extraction for further analysis?

For secondary metabolite estimation, the crude extract of A . flavus was obtained by extracting the culture filtrate with ethyl acetate. What must be the suitable solvent for suspending the...

07 December 2014 2,135 4 View