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Questions related from Kartikeya Rajpal
I need to study the cell morphology of a fungal hyphae, specifically cell wall and cell membrane. Which technique is better - confocal microscopy or Total internal reflection fluorescence...
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How does NaCl affects the metabolism of a fungi? NaCl up to a certain concentration promotes fungal growth whereas at higher concentration inhibits it. Can anybody elaborate on the mechanism...
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Is there a way of visualizing the intracellular localization of fungal secondary metabolite?
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I have tried 2 protocols for protease assay but both have given no results. Can someone please suggest a tried and tested protocol for determination of fungal protease activity?
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I have a fungal isolate of which I need to do the secondary metabolite profiling. Which of the two analytical techniques should I go for - HPLC or LC-MS? Should I directly go for the LC-MS of the...
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For secondary metabolite estimation, the crude extract of A . flavus was obtained by extracting the culture filtrate with ethyl acetate. What must be the suitable solvent for suspending the...
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