What is the best value luminescence/fluorescence microplate reader on the market?

07 August 2019 1,289 3 View

How is detection of E-cadherin in osteosarcoma cell lines U20S or Saos2?

We are conducting experiments to detect inhibition of migration in U2OS and Saos2. We detect an increase in e-cadherin mRNA but we are not able to detect the corresponding e-cadherin protein on...

03 April 2018 9,717 1 View

Human cell line overexpressing DNMT1

04 May 2017 4,818 0 View

MiRNA expression and knock-down in nude mice

04 May 2017 1,084 4 View

Is the TP53 gene entrielly deleted in the osteosarcoma cell line Saos-2?

01 February 2017 7,296 1 View

MIgration assay with U2OS, are there any tricks?

Hy everyone. We are running migration and invasion assays with U2OS cells following basic protocols from literature. We have used HGC-27 cells and it works fine but with U2OS cells we see very...

08 September 2016 5,014 4 View

Does anyone know of a SP1 null cell line?

11 December 2014 3,171 0 View

What are the statistics of success in the use of stem cells in veterinary medicine, specially for dogs? Is there data about it?

What are the cases of success versus non success

06 July 2013 6,358 1 View

Does anyone know if there is company which sells antibodies against Ehrlichia species?

I need antibodies for FACS experiments using ehrlichia infected cell culture cells.

02 March 2013 9,819 2 View

I am about to start a new project with canine cells. In my lab we have DH82, D17 and MDCK. Can anyone provide more lines?

Canine cells for a research projetct

31 December 2012 6,806 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View