Protein concentration in EMSA reaction?

11 December 2016 5,453 3 View

Sequences of pGWB3 vector with GUS reporter?

Can any one provide me the complete sequence of pGWB3 plant expression vector?

04 May 2015 10,000 4 View

Why does reaction volume decreases after restriction digestion of plasmid?

07 August 2014 8,250 4 View

How to design Real time PCR primers?

06 July 2014 3,330 8 View

How can I activate my oncogene of interest in plants?

I want to silence an oncogene (which is transfected) for a period of time and activate it. How can I do that? Could you suggest some papers for the above case,if any? This is a general case.

10 November 2013 3,699 1 View

What kind of expression and purification system can be used?

My protein of interest (human) contains glycosylation and having 34 kDa molecular weight. For crystallization purpose.

10 November 2013 2,911 1 View

Does anybody know the plant hormones which are secreted at the time of fruit formation in plants?

I am specifically interested in plant hormones which are either only secreted at the time of fruit formation or which are down regulated at that time. No need of specificity, can be any plant.

10 November 2013 9,946 12 View

Which ribonucleic acid sequence gives better results in RNA interference mediated gene silencing in plants?

I would like to know which sequence (shRNA, siRNA etc) is appropriate for gene silencing in plant species? What are the factors that should be considered for selection of RNA sequence?

10 November 2013 8,654 2 View

What are the different RNA interference methods?

I would like to know more on their advantages, disadvantage and their use in gene expression analysis.

10 November 2013 7,290 0 View

What are the best methods to evaluate and compare the gene expression in plants?

I want to check the gene expressions of two family of plants. What are the methods that I can use? I also want to know the limitations. A discussion on this topic is expected.

10 November 2013 2,434 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View