AmCube/FootWorksPro data extraction?

Has anyone got any experience of using the Amcube pressure plate with Footworks Pro software? I have a load of static standing balance measurements that the software provides (movement of centre...

27 December 2020 7,161 1 View

Can you recommend a task-based material that can be used to compare with the currently used textbooks in English teaching?

Hello everyone, It was my first experience for a research. I plan to explore the differences in grammar teaching in the two kinds of books: one is task-based and one is currently used in a...

29 October 2020 5,495 4 View

Any difference in model selection between SAS and R?

Hello all, We are working on a project as a team and some of us using R and I am using SAS. For some reason, we are getting different best models using forward and stepwise methods. I am getting...

10 November 2018 7,179 1 View

Minimum clinically important difference (MCID) and sample size calculation for interventional studies

Hi all, Would you please advise me how to use the "minimum clinically important difference - MCID" to calculate sample size for interventional studies? Are there any web based sample size...

18 May 2017 4,868 8 View

Is it right to calculate O/C ratio from deconvolved peak areas of C1s peak?

I am performing graphene oxide reduction on SiO2 substrate. In this case, the XPS spectra will measure C1s, O1s and Si2p. Due to the O in SiO2, the O/C ratio cannot be simply calculated from C1s...

08 February 2017 7,301 6 View

How can I validate the purity of GST-tagged protein?

18 December 2016 1,769 9 View

Is there any paper about calculating mass loss of reinforcements in steel fiber reinforcement concrete by corrosion current density?

My research is about the effect of steel fiber reinforced concrete with transitional reinforcements. I could only find the relationship between corrosion current density and mass loss of...

11 December 2016 7,554 0 View

Why it keeps popping out 'sequence stats calculation failed' while completing the upload of sequence data to MG-RAST?

I was uploading the sequence data to the MG-RAST server, and the notice that sequence stats calculation failed kept popping out when the upload process was about to complete, meanwhile the...

02 August 2016 932 4 View

Does anyone have idea why shRNA and SiRNA show opposit effects on immune response on RAW264.7 cells?

Hello everybody, I am trying to produce ShRNA stable cell on RAW264.7. I succeed to get cells which is around 70-80% knockdown in puromycin selection condition. But the thing is when I measure the...

22 May 2016 7,188 6 View

Culturing EOL-1 cell line (a human eosinophil cell line)?

Dear all, I would like to ask for your advice with culturing culture Eol-1 cells (eosinophilic cell line). I resuscitated cells from frozen stock and suspended in RMPI 1640+ 10% FBS + 1% PS. After...

01 May 2016 6,653 8 View

Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

Are two text files obtained separately from qiime 2 can be used together to make diversity graphs?

I have two sets of 16s microbiome sequencing data. The animal model, facility, diet vendor are the same in both sets. The only change is in the treatment. For analysis, can I use both the...

29 December 2020 6,976 3 View

What statistical modeling or correlation studies can be done for a quantitative dependent variable and qualitative independent variable?

I have to do modeling to find the correlation between my quantitative and qualitative variables. The qualitative variables cannot be classified as categorical. Is PCA or PCoA the correct choice?

07 December 2020 5,444 3 View

How to use Vegan::capscale to calculate and plot distance based- redundancy analysis (db-RDA) and add correlation cut-offs in the results?

I have a dataset with relative abundances of Genomes against their COG-ids. It is a large data frame with 4214 rows (COG-ids) and 1313 columns...

13 September 2020 2,334 1 View

Can anyone help me to Analysis rRNA Gene sequences with QIIME2?

I got the Illumina paired-end 16S sequences results. I tried to analysis them with QIIME2, but unfortunately, I found myself stuck many times. Can anyone help me,and how much will it cost? Please...

02 August 2020 467 5 View

How can I know which family/species of virus is making the difference between my samples?

I have several metagenomic samples, from which I got the viromes through a bioinformatics pipeline. I made a PCoA of those samples, and some of the samples cluster together. I would like to know...

08 July 2020 8,434 2 View

Am using software STAMP, to compare the gut microbiota of two groups. I need to know what is the significant different in family level ,NGS,QIIME?

Hi All I start sing the software STAMP, My project is to compare two gut microbiota groups. I need to know what is the significant different between this tow group in family level. could you...

17 November 2019 2,650 2 View

Importing paired FASTQ file into qiime had errors. any help?

Hi. I activated qiime 2 2019, prepared a folder named '6 sample file' to try data import which has metadata, manifest, and 6 data sequences as in the picture. I received this error. maybe as...

21 October 2019 5,132 2 View

How to convert files into .qza for qiime analysis? and how to prepare/find metadata/ mapping file?

I prepared manifest file using all FASTQ sequences and now I need to prepare metadata file. Is it a must? Also, how to convert manifest file (csv) into .qza My facility gave me a yml file with...

09 October 2019 6,446 1 View

Importing files into qiime 2 not working. any clue?

I am watching qiime importing metadata tutorial. I downloaded the barcode and sequence file using url and tried to open in qiime but they do not work as follow: (micro) a-PC:desktop hebahussein$...

09 October 2019 683 3 View