What does it mean that the closer or, the more evenly distributed the sample points during PCoA for 16sr RNA sequencing analysis? Is there any relation about the diversity of gut microbes among the treatment groups? What do the higher or lower values of PCA1 and PCA2?
Hello!
In PCoA plots, higher or lower values according to certain axes are not so important. One should pay attention to point clusters or groups. If you have different treatments, it is a good idea to mark data points according to them by color, size, or shape. In that way, you can see if there are differences between treatments by observing if samples were grouped by treatments or by some of them. One also can use PERMANOVA and PERMDISP to check if the distances between groups of samples or their distributions differences are significant.
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