I have been trying to detect DYRK1A in 293 cell lysates by Western Blotting but I cannot get any clear bands. I am using DYRK1A/B Antibody - #DF10329. I haven't tried to heat shock the cells but I have tried starving the cells.
Whoever has an electronic version of this book, sell it to me,《potato handbook crop of the future》Thanks
15 June 2024 4,404 0 View
《Plant Nutrition Diagnostics: Potato》
03 June 2024 1,663 2 View
I want to get a clear background DIG-ISH. I cloud trust that the DIG- riboprobes are right, and they are not degrdation about my riboprobes. I couldn't confirm it is no signal or a endogenous...
23 March 2023 9,884 0 View
I need information about Amazon logistics
03 January 2023 4,827 2 View
Can anyone help me discuss how logistics in developing countries are going now
21 December 2022 888 3 View
I am new to this field. Many EIS graphs are one semicircle or two semicircles. Why does the EIS graph I tested have a small tail on the left? How should this graph be analyzed?
02 August 2022 7,806 4 View
Hello everyone. I want to do trypsin digestion for peptides sample preparation in LC/MS. But my laboratory doesn't have DDT (Dithiothreitol). Can I use b-mercaptoetanol in stead of DDT for...
07 July 2022 9,032 1 View
how to export local damage data using postResult for view in paraview software?
18 April 2022 8,163 5 View
Hello everybody, My research is related to kinetic facade for building energy efficiency. I am using EnergyPlus to do simulations at the moment. I also want to study how my facades work in future...
09 May 2021 702 16 View
I extract dna from duck whole blood. Blood samples are preserved in edta. After adding protein k and lysis solution, blood is coagulated. It make me unable to extract. I don't know why. It's my...
23 April 2021 5,461 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
for eg. transition shock scale
01 August 2024 5,998 0 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View
I have this problem with my western blot for the ABCG2 antibody on nitrocellulose membrane. Help please
02 July 2024 8,715 2 View