Get PMID from PubMed automatically?

Dear experts, I got first author, publication year, journal, and paper title in separate columns of a Google Sheet. Are there ways to crawl PubMed and automatically get the PMIDs of the listed...

23 December 2020 3,949 4 View

[HBV DNA] Getting smear instead of bands. Why?

I extracted HBV DNA from serum (frozen, stored at -80 degree). I used Qiagen's HotStarTaq Plus PCR Kit to amplify the region encompassing the preS1, preS2, S gene. the product is 1373bp long.The...

25 May 2020 8,715 1 View

How can I extract all-time Twitter contents using keywords, without Premium or Enterprise API?

I would like to analyse Twitter contents, the words in the mentions, the locations of the Tweets, how many re-tweets and favorites, the trending, etc, based on keyword searches. I see that many...

06 April 2020 5,531 5 View

How to treat Time varying covariates in HLM analysis?

Hello, i'm currently running an analysis of longitudinal data. i'm using HLM analysis using the SAS software. the design is as follow: IV1 - time (3 time points: before\during\after stress) -...

28 November 2019 7,212 2 View

How to apply fuzzy K-means with spatial constraints in R?

Hello. I am looking for a solution in R to apply spatially-constrained clustering using fuzzy K-means (fuzzy c-means). I came across the spatialClust package, and was wondering whether anyone may...

30 October 2019 4,701 2 View

Methods for measuring N-mineralization in field sites/experiments?

A student is installing a field trial that we expect to largely impact soil moisture and temperature, and we simultaneously want to measure the secondary impacts on N-mineralization in the field....

26 March 2019 4,138 3 View

What to do if the assumption of Homogeneity of variance has been violated - Three-way anova?

i have a 3-way mixed anova design: 2 IVs that are within-subject and 1 IV's that is a between-subject variable. the assumption of equality of variance (Leven's Test) was violated, as the test came...

18 August 2017 3,240 4 View

Could I have a 50-75bp band for primer dimers, even if my primers are around 20 bp long?

Hi I have problems with my PCR negative controls, Sometimes I see a band in the gel electrophoresis in these controls (same master mix that in samples but without DNA template). The band is...

14 June 2017 4,540 3 View

How to find the evidence of N-A-S-H phase of slag/fly ashgeopolymer ?By XRD or NMR or anything else?

24 September 2016 9,339 3 View

Dynamic characteritisc of fluid bearings/seals due to angular perturbation?

When determining the stiffness and damping coefficients for a journal or thrust bearings/seals with computational fluid dynamics (CFD) method, the typical consideration from the literature is to...

31 July 2016 6,581 3 View

What statistical modeling or correlation studies can be done for a quantitative dependent variable and qualitative independent variable?

I have to do modeling to find the correlation between my quantitative and qualitative variables. The qualitative variables cannot be classified as categorical. Is PCA or PCoA the correct choice?

07 December 2020 5,444 3 View

How to use Vegan::capscale to calculate and plot distance based- redundancy analysis (db-RDA) and add correlation cut-offs in the results?

I have a dataset with relative abundances of Genomes against their COG-ids. It is a large data frame with 4214 rows (COG-ids) and 1313 columns...

13 September 2020 2,334 1 View

How can I know which family/species of virus is making the difference between my samples?

I have several metagenomic samples, from which I got the viromes through a bioinformatics pipeline. I made a PCoA of those samples, and some of the samples cluster together. I would like to know...

08 July 2020 8,434 2 View

Running multi-locus SSR binary data on GenAlEx?

I have data from SSR markers that have 2-10 bands each. I was able to run analysis wherein the program consider each band as single loci. The program gave me the following data (as in attached...

24 February 2020 6,318 1 View

How to perform PCoA in R with given UniFrac distance matrix?

I have a distance matrix (weighted UniFrac distances) and I want to perform PCoA Analysis in R. I tried: beta

26 September 2019 5,762 2 View

How to perform PCoA in R with given UniFrac distance matrix?

I have a distance matrix (weighted UniFrac distances) and I want to perform PCoA Analysis in R. I tried: beta

08 September 2019 2,363 2 View

Principal Coordinates (PCoA) or Discriminant Analysis (LDA)?

Hi, I am a botanist and in my experience, LDA always finds better differentiation than PCoA among a-priori defined groups (for me = taxa). I always use PCoA for molecular datasets but for...

02 March 2019 5,062 3 View

Principal Coordinates (PCoA) or Discriminant Analysis (LDA)?

Hi, I am a botanist and in my experience, LDA always finds better differentiation than PCoA among a-priori defined groups (for me = taxa). I always use PCoA for molecular datasets but for...

28 February 2019 5,430 3 View

What is the preferable way to calculate explained variance of a pcoa axis, if pcoa contains negative eigenvalues?

Hi all, I have a principal coordinate analysis of a distance matrix (pairwise simpson dissimilarity matrix) with a lot of negative Eigenvalues (some of them quite high). I want to calculate the...

20 February 2019 3,363 2 View

Is there any software that can analyze Genetic diversity parameters for SRAP markers with multiple loci? I have tried PowerMarker and GenAlEx. But?

In checking for genetic diversity parameters such as the proportion of polymorphic loci (P), the average number of alleles per locus (Ap), the average number of effective alleles per locus (Ae),...

31 December 2018 3,979 2 View