Hey everyone,
Has anybody might have encountered "unexpected error (13)" when trying to run PCoA test in GenAlex and knows how to fix it? (input - PhiPTP, AMOVA results).
Thank you kindle,
Noa
Hi everyone, I am currently working on RNA extraction on tissue, but unfortunately, I only have large frozen clinical tissue on hand. In order to maximize the RNA integrity, I plan to use...
25 June 2024 7,550 1 View
Hi, I am currently working on RNA extraction on blood serum samples using Trizol. However, the RNA yield is quite bad, on average, only yielded 200ng per 200ul of sample input, and the A260/280...
18 June 2024 6,853 3 View
I am trying to do the affinity purification of MS2-tagged mRNA by capturing it on GST-MCP (MS2 coat protein) immobilized on glutathione beads. I used two approaches: transfecting the construct...
12 June 2024 504 0 View
Hi, I am currently looking for a reliable method to extract miRNA from cell and serum samples. I have read some papers stating TRIzol is suitable for both samples, but the result highly depends...
24 April 2024 3,995 3 View
Hi, I am currently looking for suggestions on choosing which cryovial to use for cell cryopreservation, internal or external threaded, do you guys have a preference? or is there any reason for...
11 March 2024 1,587 1 View
I am currently doing miRNA research and got the miRCURY LNA RT kit from Qiagen for RT reaction to turn my RNA into cDNA. A few papers have used SYBR green kit from another brand and not the...
10 March 2024 351 1 View
Hi guys, I am currently working on qRT-PCR. However, the amount cDNA I got from RT reaction is too little to assess all the targets. I saw some papers and websites mentioning amplifying cDNA with...
29 February 2024 135 3 View
A error code related to biacore 3000 autosampler showed up after I ran a new template and the needle was inserted into wrong position while filling sample. The needle still works fine during...
06 February 2024 1,747 0 View
As per the tiltle said, I want to qualitatively test how much IAA can be produced by some of the bacteria that I have just isolated from a soil sample. After culturing the soil bacteria in a...
03 December 2023 4,407 1 View
I am currently exploring the effect of post-translational modifications (PTMs) on the activity of ABA receptors (PYR/PYL/RCAR) in Arabidopsis, with a specific emphasis on the presence of cysteine...
11 February 2023 136 1 View
Hi, I'm new to bioinformatics. I have a weighted UniFrac metric generated from some bacterial samples and I want to test the effect of two factors A and B (both binary) on the composition using...
25 March 2024 9,618 1 View
I want to study the genetic diversity and population structure of my samples using SSR markers. The PCR products of my samples show many bands of different sizes. and i am confused about how to...
06 March 2024 3,177 1 View
Please help me understanding the difference in Binary Diploid, Binary Haploid, and Haploid data types in GenAlex 6.5. I want use a binary dataset (RAPD) from fungal species which has a haploid...
14 February 2024 9,257 3 View
I am currently working on some plant species ISSR scoring data, which consists of 0s and 1s. I would like to perform a Principal Coordinates Analysis (PCoA) using GenAlEx. Could someone guide me...
07 January 2024 4,204 1 View
I need to calculate number of different and effective allele for a diversity study using large SNP dataset. Normally I use GenAlex but I can't load the data due to limited no of columns. Please...
01 January 2024 6,128 2 View
What does it mean that the closer or, the more evenly distributed the sample points during PCoA for 16sr RNA sequencing analysis? Is there any relation about the diversity of gut microbes among...
28 October 2023 3,227 1 View
How do I prepare data from an issr marker gel for analysis with the GenAlex program, and how do I determine the number and percentage of polymorphic loci, the average and effective number of...
08 November 2022 8,941 3 View
Hello everyone, As a PhD student in paleontology, some fossil specimens may not be accessible and only visible via pictures from publications. In order to perform some analysis (such as linear...
20 October 2022 1,298 6 View
Does anyone have tips or recommendations for importing aligned sequences to GenAlEx? I keep getting an error message that says my file does bot conform to the Nexus format. I can get GenAlEx to...
17 October 2022 9,530 0 View
We did analysis of the probability of identity (PID) in GenAlEx to see the likelihood that sexually derived individuals can be discriminated using eigth loci. Were we right on the interpretation...
16 October 2022 3,470 0 View