I want to get a clear background DIG-ISH. I cloud trust that the DIG- riboprobes are right, and they are not degrdation about my riboprobes. I couldn't confirm it is no signal or a endogenous alkaline phosphorylase caused reslut.It's all purple on my section slids. I use the Rohe production,including Blocking Reagent,antibody-Fab,BCIP/NBT .please help me. I don't know why, it confuse me for a long time.

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