Insect cuticle is composed of a mixture of protein, chitin and lipids; hence,entomopathogenic fungi must produce several enzymes to penetrate this barrier. Chitinases are broadly classified as endo- and exochitinases. The endochitinase activity is defined as the random cleavage at internal points in the chitin chain. The exochitinase activity is defined as the progressive action starting at the non-reducing end of chitin with the release of chitobiose or N-acetylglucosamine units.
Hi, The enzymes including both exo-- and endo- are used to complete digestion of a substrate. If you get to understand complete hydrolyzing of a compound you must measure both classes. Regarding virulence, one of them might be sufficient.
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Dear farzaneh sadat Seyedtalebi
I had been appllied , the effect of two types of chitinase at antagonisms between promoting and pathogenic fungi , the results answered , that the two types are affected , but the endo was more one
ارزو ميكنم اين باسخ بفائدة شما ميكوند
Hello Dear
Penetration of an entomopathogenic fungi is the first and the most impotrant of infection. After penetration, other fungi metabolits such as protein toxins casued diseases. After penetration the fungi are activated and caused infection. So enzymes that affect the primary penetration are more important than. I studied entomopathigenic fungi epizootiology on Oryzaephilus surinamensis population. Please follow my publications. Thanks a lot.
Dear Farzaneh
salam arz mekonam..I think that endochitinase more important because it degradade and Catalysis of the hydrolysis of nonterminal (1->4)-beta linkages of N-acetyl-D-glucosamine (GlcNAc) polymers of chitin and chitodextrins. Typically, endochitinases cleave randomly within the chitin chain. but it work together well
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