Though many reports says all parts of the plant contains L-DOPA when I had estimated the leaf alone it shows no presence of L-DOPA and same with the callus developed from the leaves.
Any idea how to proceed?
Thanks
The best method is HPLC. L-DOPA and dopamine are easily detected at 272 nm
You can quantity the same using HPLC analysis or LCMS
Article Elicitors and precursor induced effect on L-Dopa production ...
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