21 Questions 9 Answers 0 Followers
Questions related from Rakesh Bhaskar
In natural sources what is the purity of L-DOPA and does that purity remain same in callus or cell culture?
23 February 2022 895 0 View
When 100g dr weight was taken and using soxhlet method methanolic extract was prepared. The yield was 60g. I was quite amused by the values, is this high range value possible? Thank you
28 January 2022 9,251 4 View
My work is related to induction of callus and optimization of cell suspension culture of Mucuna to produce L-DOPA.
01 December 2021 8,355 2 View
Does activated charcoal when added to MS media adsorb hormones and vital nutrients from media and affect plant growth? Because charcoal has absorbing activities. It is used to adsorb phenolics...
27 June 2021 2,254 2 View
L-DOPA is found many folds high in seeds but people choose leaf as an explant for inducing callus and quantification. Instead if seed is chosen the callus induced from seed will have higher...
03 April 2021 2,478 0 View
For eg, if a metabolite is produced high in seeds, should seed be taken as a explant must and should. If leaf or stem is taken as an explant and callus induced will the conc. Of the metabolite...
03 April 2021 6,691 0 View
I'm working on hairy root culture in Mucuna pruriens with two strains MTCC and KCTC which are very old cultures. I'm unable to induce hairy root culture. Any one is working on same plant? Thank you
20 January 2021 8,366 8 View
Though many reports says all parts of the plant contains L-DOPA when I had estimated the leaf alone it shows no presence of L-DOPA and same with the callus developed from the leaves. Any idea how...
17 November 2020 7,342 3 View
I would be happy to get your contact details those working on the above mentioned study as I want to have some discussions and clear my doubts. Thank you 🙏
16 November 2020 8,822 1 View
For eg, when I'm doing protein assay by Lowry's method, should the total volume of my standards and my unknown sample be same?
16 November 2020 5,762 3 View
plant extract stock is 10 mg/mL from which I pipetted 20, 40, 60, 80, 100, 200 uL and made up to 3 mL using methanol then i added 1 ml of 0.004% dpph solution (dissolved in methanol) incubated in...
03 September 2020 1,371 2 View
If chemical process what are its disadvantages. If a plant system(in-vitro) is produced with high L-DOPA content how feasible will it be to use them? Will it be really helpful for the pharmacy...
10 June 2020 7,895 0 View
Bacteria infects all sort of living organisms. Can it infect virus when virus is alive inside the host? If not why is that not possible?
27 April 2020 9,731 5 View
I'm growing mucuna seeds invitro. I soaked mucuna seeds in 1% ascorbic acid for 3 hours and then inoculated in basal media with 1 mg/L GA3. Though it's germinating, media is turning black....
29 January 2020 1,536 5 View
For eg. If some important metabolite is found only in seeds, and if I take the leaves of the seeds grown in pots and initiate callus culture on one hand, and on the other if I germinate the...
29 January 2020 1,056 3 View
It dissolved in 60-65% ethanol but after few hours if storage at 4 degree Celsius it is forming precipitation
20 January 2020 5,219 9 View
Is there any other method for SA stock preparation
16 January 2020 6,631 4 View
GA3 is added for germinating seeds in-vitro but should it be added before or after autoclaving
16 January 2020 2,391 7 View
Plasmids are mainly helping in antibiotic resistance and sometimes in replication. But why are they present as a separate entity in bacterial cells?
11 December 2019 263 3 View
Alkaline lysis method
11 December 2019 2,646 2 View
Dear Friends, I have completed my PhD in Botany (Plant Biotechnology - Tissue culture) and Im keenly looking forward to continue my research career as a Post-Doctoral fellow in any reputed...
01 January 1970 2,209 0 View
