Does HCV - NS4B give 2 bands on western blot ?

09 October 2015 5,310 3 View

How long do Huh7.5 cells take to reach 100 % confluency ?

08 September 2015 552 4 View

Could MALDI TOF - MS be used as a specific and sensitive strain- level identification tool for Staphylococcus aureus ?

02 March 2015 2,042 9 View

How many isolates are needed to do a "multicenter study " ?

i am about to make a survey for a large number of bacterial clinical isolates for a specific type of antibiotic resistance ................... these isolates are collected from many health...

31 December 2014 7,909 2 View

How can I perform a Coagulase test for the detection of Staphylococcus aureus ?

09 October 2014 9,985 13 View

What is the difference between "the Abstract" and " the conclusion" ?

in review articles what is the main differnce between "abstract " and "conclusion " ....... what elements each of them should or shouldn't contain ?

07 August 2014 668 32 View

Can I use human plasma for coagulase testing?

I want to do a coagulase test for the identification of staphylococcus aureus. When I asked how to get the "rabbit plasma " and what is the most appropriate procedure for doing so, someone...

05 June 2014 1,242 7 View

How can I get a standard Vancomycin resistant staphylococcus aureus strain?

I want to get a fully Vancomycin resistant staphylococcus aureus strain. I checked the ATCC and found that they offer only intermediate resistant strains ? Any suggestions for alternative sources?

05 June 2014 7,536 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View