Mohamed Medhat Samir Shamseldin

9 Questions 10 Answers 0 Followers

Questions related from Mohamed Medhat Samir Shamseldin

Which kind of samples could be used for ELISA detection of anti-nuclear antibodies(ANAs)?is it ok to use plasma or serum?

i am going to use AESKLISA...

09 September 2018 8,729 9 View

Does HCV - NS4B give 2 bands on western blot ?

i am working with a set of plasmid constructs for HCV proteins ; each one is separately loaded with individual FLAG-tagged protein ............ after transfecting the NS4B containing plasmid in...

10 October 2015 5,395 3 View

How long do Huh7.5 cells take to reach 100 % confluency ?

i am about to seed a T75 bottle with different dilutions ( 1:2 and 1:10 ) of 100% confluent Huh7.5 cells(about 7-8X10 5 cell /ml ) ............ how long do those seeded cells take to reach 100...

09 September 2015 658 4 View

Could MALDI TOF - MS be used as a specific and sensitive strain- level identification tool for Staphylococcus aureus ?

i want to use MALDI -TOF biotyper to confirm the identity of a number of staph.aureus clinical isolates and i read a review that claimed its ability to provide a strain-level identification of...

03 March 2015 2,172 9 View

How many isolates are needed to do a "multicenter study " ?

i am about to make a survey for a large number of bacterial clinical isolates for a specific type of antibiotic resistance ................... these isolates are collected from many health...

01 January 2015 7,993 2 View

How can I perform a Coagulase test for the detection of Staphylococcus aureus ?

I am about to perform the Coagulase test for detection of Staphylococcus aureus using citrated human plasma. Do you have any advices or recommendation regarding the best conditions, dilutions,...

10 October 2014 10,084 13 View

What is the difference between "the Abstract" and " the conclusion" ?

in review articles what is the main differnce between "abstract " and "conclusion " ....... what elements each of them should or shouldn't contain ?

08 August 2014 742 32 View

Can I use human plasma for coagulase testing?

I want to do a coagulase test for the identification of staphylococcus aureus. When I asked how to get the "rabbit plasma " and what is the most appropriate procedure for doing so, someone...

06 June 2014 1,351 7 View

How can I get a standard Vancomycin resistant staphylococcus aureus strain?

I want to get a fully Vancomycin resistant staphylococcus aureus strain. I checked the ATCC and found that they offer only intermediate resistant strains ? Any suggestions for alternative sources?

06 June 2014 7,615 4 View