The transfected cells are showing very weak mcherry fluorescence. How to detect mcherry expression?

The animal cells are transfected with a vector having mcherry reporter gene. The transfected cells show very weak fluorescence. How to get a good quality fluorescener image showing mcherry...

07 August 2018 7,498 3 View

Why could be there a redshift of emission peak of a dried fluorescent dye as compared to the solution?

A fluorescent dye has emission peak at 575 in aquous solution but is shifted to 615 upon drying. what could be possible reasons?

04 May 2018 6,545 1 View

Which cloning cylinders are best for isolating cell colonies? Glass or pyrex?

For isolating cell colonies cloning cylinders made of glass or pyrex are most easy to handle and preferred?

31 December 2017 4,934 6 View

How to preserve stably transfected animal cell lines?

Can stably transfected cell lines be cryo preserved using general preservation protocol?

31 December 2017 6,560 5 View

Which laser suitable for activation of ChR2 protein in mammalian cells?

Which laser is most suitable for optogenetic activation of ChR2 in non-neuronal mammalian cells. What power range is required?

31 December 2016 8,618 4 View

Sources for Optogenetic ChR2 mammalian expression vectors?

I am interested to get ChR2 mammalian expression vectors. Please tell what are the sources I can get hese from. One I know is Addgene. Kindly inform if there are any other too...

11 December 2016 962 1 View

What precautions and safety guidelines to be followed for staining samples with Uranyl acetate for TEM?

What precautins to be taken and how to discard the material stained with uranyl acetate safely?

10 November 2016 9,634 7 View

How can cell lines be ordered from SIGMA?

I want some cell lines from SIGMA, but their online lists just shows about the DNA , cDNA or RNA of the respective cell line. How can I order the cells in culture?

10 November 2016 5,882 2 View

Can optogenetic vectors be shuttled between bacteria and mammalian cells ?

09 October 2016 3,267 1 View

Please provide a reference for thermal conductivity of polystyrene sulfonate?

I need to know the thermal conductivity of polystyrene sulfonate.

03 April 2016 4,546 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

No title

02 March 2021 3,060 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View