I am interested to get ChR2 mammalian expression vectors. Please tell what are the sources I can get hese from. One I know is Addgene. Kindly inform if there are any other too...
https://www.researchgate.net/post/Where_do_you_order_ready-to-use_AAV_GCaMP6_virus
The animal cells are transfected with a vector having mcherry reporter gene. The transfected cells show very weak fluorescence. How to get a good quality fluorescener image showing mcherry...
07 August 2018 7,663 3 View
A fluorescent dye has emission peak at 575 in aquous solution but is shifted to 615 upon drying. what could be possible reasons?
04 May 2018 6,686 1 View
For isolating cell colonies cloning cylinders made of glass or pyrex are most easy to handle and preferred?
31 December 2017 5,074 6 View
I want to stably transfect Min6 cell lines with ciruclar plasmid . Kindly suggest which method is the most efficient.
31 December 2017 9,511 2 View
Can stably transfected cell lines be cryo preserved using general preservation protocol?
31 December 2017 6,705 5 View
Which laser is most suitable for optogenetic activation of ChR2 in non-neuronal mammalian cells. What power range is required?
31 December 2016 8,772 4 View
I want some cell lines from SIGMA, but their online lists just shows about the DNA , cDNA or RNA of the respective cell line. How can I order the cells in culture?
10 November 2016 6,044 2 View
What precautins to be taken and how to discard the material stained with uranyl acetate safely?
10 November 2016 9,775 7 View
Optogenetic vectors are expression vectors for mammalian cells. But to preseve and store them or multiply, can these be transferred to bacterial strains?
09 October 2016 3,408 1 View
I need to know the thermal conductivity of polystyrene sulfonate.
03 April 2016 4,700 1 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View