The transfected cells are showing very weak mcherry fluorescence. How to detect mcherry expression?

The animal cells are transfected with a vector having mcherry reporter gene. The transfected cells show very weak fluorescence. How to get a good quality fluorescener image showing mcherry...

07 August 2018 7,498 3 View

Why could be there a redshift of emission peak of a dried fluorescent dye as compared to the solution?

A fluorescent dye has emission peak at 575 in aquous solution but is shifted to 615 upon drying. what could be possible reasons?

04 May 2018 6,545 1 View

Which cloning cylinders are best for isolating cell colonies? Glass or pyrex?

For isolating cell colonies cloning cylinders made of glass or pyrex are most easy to handle and preferred?

31 December 2017 4,934 6 View

Which is the most efficient method of transfection of Min6 cell lines with circular plasmid? Calcium phosphate/lipofectamine/electroporation or other?

I want to stably transfect Min6 cell lines with ciruclar plasmid . Kindly suggest which method is the most efficient.

31 December 2017 9,333 2 View

How to preserve stably transfected animal cell lines?

Can stably transfected cell lines be cryo preserved using general preservation protocol?

31 December 2017 6,560 5 View

Which laser suitable for activation of ChR2 protein in mammalian cells?

Which laser is most suitable for optogenetic activation of ChR2 in non-neuronal mammalian cells. What power range is required?

31 December 2016 8,618 4 View

What precautions and safety guidelines to be followed for staining samples with Uranyl acetate for TEM?

What precautins to be taken and how to discard the material stained with uranyl acetate safely?

10 November 2016 9,634 7 View

How can cell lines be ordered from SIGMA?

I want some cell lines from SIGMA, but their online lists just shows about the DNA , cDNA or RNA of the respective cell line. How can I order the cells in culture?

10 November 2016 5,882 2 View

Can optogenetic vectors be shuttled between bacteria and mammalian cells ?

09 October 2016 3,267 1 View

Please provide a reference for thermal conductivity of polystyrene sulfonate?

I need to know the thermal conductivity of polystyrene sulfonate.

03 April 2016 4,546 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View