The animal cells are transfected with a vector having mcherry reporter gene. The transfected cells show very weak fluorescence. How to get a good quality fluorescener image showing mcherry expression? How to identify mcherry expresssion?
Are you trying to image live animals, or a cell line from animal cells? Low fluorescence in animals could be due to your cells not being transfected efficiently. I would use in vivo transfection reagents for the tissue type (or generics) that you are targeting (e.g. https://altogen.com/product/nanoparticle-in-vivo-transfection-reagent/). mCherry expression is usually quantified through spectrophotometry. If you're doing the transfection of a cell line, then make sure to use cell-line specific transfection reagents. In either case, raising your transfection efficiency should make the identification process easier.
Thanks.....I am trying to visualise transferred cell line by fluorescence microscopy
I would make sure to use a filter for mCherry, because that helps with the clarity. Read this study (Article Fluorescence Fluctuation Spectroscopy of mCherry in Living Cells
) and that should help you identify possible issues with your protocols.- Badges
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