The transfected cells are showing very weak mcherry fluorescence. How to detect mcherry expression?

The animal cells are transfected with a vector having mcherry reporter gene. The transfected cells show very weak fluorescence. How to get a good quality fluorescener image showing mcherry...

07 August 2018 7,498 3 View

Why could be there a redshift of emission peak of a dried fluorescent dye as compared to the solution?

A fluorescent dye has emission peak at 575 in aquous solution but is shifted to 615 upon drying. what could be possible reasons?

04 May 2018 6,545 1 View

Which is the most efficient method of transfection of Min6 cell lines with circular plasmid? Calcium phosphate/lipofectamine/electroporation or other?

I want to stably transfect Min6 cell lines with ciruclar plasmid . Kindly suggest which method is the most efficient.

31 December 2017 9,333 2 View

How to preserve stably transfected animal cell lines?

Can stably transfected cell lines be cryo preserved using general preservation protocol?

31 December 2017 6,560 5 View

Which laser suitable for activation of ChR2 protein in mammalian cells?

Which laser is most suitable for optogenetic activation of ChR2 in non-neuronal mammalian cells. What power range is required?

31 December 2016 8,618 4 View

Sources for Optogenetic ChR2 mammalian expression vectors?

I am interested to get ChR2 mammalian expression vectors. Please tell what are the sources I can get hese from. One I know is Addgene. Kindly inform if there are any other too...

11 December 2016 962 1 View

What precautions and safety guidelines to be followed for staining samples with Uranyl acetate for TEM?

What precautins to be taken and how to discard the material stained with uranyl acetate safely?

10 November 2016 9,634 7 View

How can cell lines be ordered from SIGMA?

I want some cell lines from SIGMA, but their online lists just shows about the DNA , cDNA or RNA of the respective cell line. How can I order the cells in culture?

10 November 2016 5,882 2 View

Can optogenetic vectors be shuttled between bacteria and mammalian cells ?

09 October 2016 3,267 1 View

Please provide a reference for thermal conductivity of polystyrene sulfonate?

I need to know the thermal conductivity of polystyrene sulfonate.

03 April 2016 4,546 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to properly calculate the fluid solid heat transfer between polypropylene matrix and glass fiber in a porous medium?

I'm building a cfx model of thermoplastic pultrusion process on ansys and i have a doubt on how is the proper way to set this parameter in a porous medium

24 February 2021 1,915 1 View

How to properly calculate Interfacial area density in a porous domain?which are possible value for glass fiber?

I'm doing a cfx simulation of a multiphase flow in pultrusion and i set the Interfacial Area density in the heat exchange of a porous medium in this way IAD=Total fiber lateral surface of fiber...

23 February 2021 6,043 1 View