Rashmi Shrivastava

11 Questions 13 Answers 0 Followers

Questions related from Rashmi Shrivastava

The transfected cells are showing very weak mcherry fluorescence. How to detect mcherry expression?

The animal cells are transfected with a vector having mcherry reporter gene. The transfected cells show very weak fluorescence. How to get a good quality fluorescener image showing mcherry...

08 August 2018 7,614 3 View

Why could be there a redshift of emission peak of a dried fluorescent dye as compared to the solution?

A fluorescent dye has emission peak at 575 in aquous solution but is shifted to 615 upon drying. what could be possible reasons?

05 May 2018 6,646 1 View

How to preserve stably transfected animal cell lines?

Can stably transfected cell lines be cryo preserved using general preservation protocol?

01 January 2018 6,655 5 View

Which is the most efficient method of transfection of Min6 cell lines with circular plasmid? Calcium phosphate/lipofectamine/electroporation or other?

I want to stably transfect Min6 cell lines with ciruclar plasmid . Kindly suggest which method is the most efficient.

01 January 2018 9,456 2 View

Which cloning cylinders are best for isolating cell colonies? Glass or pyrex?

For isolating cell colonies cloning cylinders made of glass or pyrex are most easy to handle and preferred?

01 January 2018 5,030 6 View

Which laser suitable for activation of ChR2 protein in mammalian cells?

Which laser is most suitable for optogenetic activation of ChR2 in non-neuronal mammalian cells. What power range is required?

01 January 2017 8,713 4 View

Sources for Optogenetic ChR2 mammalian expression vectors?

I am interested to get ChR2 mammalian expression vectors. Please tell what are the sources I can get hese from. One I know is Addgene. Kindly inform if there are any other too...

12 December 2016 1,107 1 View

How can cell lines be ordered from SIGMA?

I want some cell lines from SIGMA, but their online lists just shows about the DNA , cDNA or RNA of the respective cell line. How can I order the cells in culture?

11 November 2016 5,992 2 View

What precautions and safety guidelines to be followed for staining samples with Uranyl acetate for TEM?

What precautins to be taken and how to discard the material stained with uranyl acetate safely?

11 November 2016 9,734 7 View

Can optogenetic vectors be shuttled between bacteria and mammalian cells ?

Optogenetic vectors are expression vectors for mammalian cells. But to preseve and store them or multiply, can these be transferred to bacterial strains?

10 October 2016 3,360 1 View

Please provide a reference for thermal conductivity of polystyrene sulfonate?

I need to know the thermal conductivity of polystyrene sulfonate.

04 April 2016 4,641 1 View