11 Questions 13 Answers 0 Followers
Questions related from Rashmi Shrivastava
The animal cells are transfected with a vector having mcherry reporter gene. The transfected cells show very weak fluorescence. How to get a good quality fluorescener image showing mcherry...
08 August 2018 7,618 3 View
A fluorescent dye has emission peak at 575 in aquous solution but is shifted to 615 upon drying. what could be possible reasons?
05 May 2018 6,648 1 View
Can stably transfected cell lines be cryo preserved using general preservation protocol?
01 January 2018 6,657 5 View
I want to stably transfect Min6 cell lines with ciruclar plasmid . Kindly suggest which method is the most efficient.
01 January 2018 9,458 2 View
For isolating cell colonies cloning cylinders made of glass or pyrex are most easy to handle and preferred?
01 January 2018 5,032 6 View
Which laser is most suitable for optogenetic activation of ChR2 in non-neuronal mammalian cells. What power range is required?
01 January 2017 8,715 4 View
I am interested to get ChR2 mammalian expression vectors. Please tell what are the sources I can get hese from. One I know is Addgene. Kindly inform if there are any other too...
12 December 2016 1,108 1 View
I want some cell lines from SIGMA, but their online lists just shows about the DNA , cDNA or RNA of the respective cell line. How can I order the cells in culture?
11 November 2016 5,994 2 View
What precautins to be taken and how to discard the material stained with uranyl acetate safely?
11 November 2016 9,736 7 View
Optogenetic vectors are expression vectors for mammalian cells. But to preseve and store them or multiply, can these be transferred to bacterial strains?
10 October 2016 3,363 1 View
I need to know the thermal conductivity of polystyrene sulfonate.
04 April 2016 4,644 1 View