Which is the correct percentage of Ethanol in the TRIZOL-RNA extraction to recover the small RNA?

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Zrinka Didara

I used always 70% ethanol for my samples but I was isolating total RNA not just small RNA. In this publication from 2016 " A microRNA isolation method from clinical samples" they state small RNAs were enriched and recovered from the supernatants by applying a combination of LiCl and ethanol.

David Otaegui

Thanks a lot Zrinka, I assume that in your total RNA extraction, small RNA are included right?

Hassan Nima

I tend to 70%......

this link is useful

https://tools.thermofisher.com/content/sfs/manuals/trizol_reagent.pdf

Regards

Yes should be also small RNA