Does anyone has an idea about the coverage needed to study CircRNA in RNA( enriched in circRNA by rRNA depletion and RNASe R enrichement) from PBMC?

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Gopal Pandi

Dear David, preferentially you have to have 100 million reads and to reach such coverage, your input RNA (after RNAse R) should be higher.

Best wishes for your research.

Francisco J. Enguita

Hi David

Difficult question. There is no specific rule to cover the whole circRNA transcriptome, but of course the higher redundancy the better. You can also try to do ribo- and polyA- RNA to specifically enrich those transcripts without polyAs and increase the yield of detection for circRNAs.

Best. Paco