Batch effect in two datasets of HUgene Affymetrix arrays.
Hi David, perhaps this link to combat will help
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0017238
I am trying to find a bioinformatic tool to analyze FASTAq coming from ITS region
31 December 2018 1,273 6 View
We have some experience in different splicing in some RNA depending of the time that the blood is in the bench waiting for the RNA extraction. We are wondering if that can occurs also with circRNA.
04 May 2018 5,169 3 View
Technical question about the RNA extraction with TRIZOL
01 February 2018 9,158 4 View
How coverage will I need in a NGS study to cover all circRNA? The sample will be RNA from PBMC, previously treated with rRNA depletion and RNAse R (to enrich the sample in circ)
03 April 2016 2,032 2 View
C9orf72 is an expansion related to some neurological diseases such as ALS or dementia.
31 December 2012 1,865 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View