Depends on what equipment you have access to. The best way - freeze fracture SEM not TEM. If there are no cryogenic SEM, the freeze-fracture TEM can work.

In my opinion freeze-fracture transmission electron microscopy would be better method for this study

Freeze-fracture TEM may and/or might be the standard procedure for lipid phase-transitions study, Arbind Kumar!

Period!

Please share your reasons, Meena Thayaparan!

Depends on what equipment you have access to. The best way - freeze fracture SEM not TEM. If there are no cryogenic SEM, the freeze-fracture TEM can work.

South Africa (SA) did not have the equipment for freeze-fracture TEM which was the standard procedure for lipid phase transition study at the time - 1995! So tried high resolution SEM (HRSEM) in conjuction with the standard procedure done  in the USA! Did not know about freeze-fracture SEM!

So, dear Tobias, WHAT is really your question then?

From your question as well as from your replies to postings of other colleagues I cannot figure out what you really want or intend with your question....anything historic? [cf. Your Re: "South Africa did not have the freeze-fracture TEM which was the standard procedure for lipid phase transition study at the time - 1995! So tried high resolution SEM in conjuction with the standard procedure done  in the USA! Did not know about freeze-fracture SEM! ]....

I am sorry, this makes no sense to me except the precise reply of Vladimir Dusevich.

Regards, Wolfgang

My question is clear and/or explicit!

I have added the words equipment for to my response!

I suspect my former supervisors - Patricia Berjak and/or Norman Pammenter - and I may have been the first to use HRSEM to study lipid phase transitions at least in SA!

The late Emeritus Professor Patricia Berjak would deserve the credit! for the idea!

I was only a technician and/or writer!

Dear Tobias,

whilst I was not following this your thread for some reason (due to my soon retirement and leaving the Lab I am working)  it seems that there has been a considerable  problem in evaluation of my reply no 7 which had been upvoted by two colleagues and meanwhile DOWNVOTED  by another unknown colleague.

I DON'T say that downvoting was by anyone in this thread but would like to explain that with my reply # 7(and certainly it is my opinion right now too) I just only wanted to say that I was following and seconding the opinion and whole answer of Vladimir Dusevich (in his reply#5).

I don't understand the downvote (which I take note of) because it seems that I was right with my answer #7 though.

As it turns out now (and as I understand it) you asked for the best technique you already knew...

(and I answered  [o.k., literally not for either of the technqiues YOU anticipated] that I accepted those  techniques Vladimir mentioned: !)  

You are / were complaining about perhaps for a certain technique  ( = )  which should have been awarded to the

AND  

.

To the Downvoter:  

Excuse my faults or errors if in your opinion there was one or were some....:

To Tobias: 

 I take an oath on HAVING NOT downvoted YOUR last two replies # 8 &9.

Unfortunately I can't proove that for you because I will NOT upvote your replies #8 & 9. but it would be easy to show that.

To the Readers:

Wish you a pleasant day, evening or morning, wherever you live...

Wolfgang

PS: It is appreciated if you turn out the truth and background of your question and don't say:  

but tell us that you have done formerly (as of your replies < some 10 years ago>, resp.) a technique/method which was not used at that time at least in SA=South Africa/ZA (= < HRSEM in conjunction with the standard freeze-fracture a decade ago> )  and that

>>YOU may have been the first to use HRSEM to study lipid phase transitions at least in SA!