What are mediators of apoptosis?

More Tobias M. Ntuli's questions See All

What is the active ingredient in tobacco?

The active ingredients in alcohol and ganja and/or marijuana are ethanol and cannabis, respectively!

05 June 2016 5,309 3 View

Which is the better method between high-resolution scanning and/or freeze-fracture transmission electron microscopy for lipid phase-transitions study?

Used high-resolution scanning electron microscopy for the study of lipid phase-transitions in desiccation-responses of wild rice in conjunction with the standard freeze-fracture a decade ago

06 July 2015 6,455 10 View

What are the differences in desiccation sensitivity and tolerance mechanisms in reproductive and non-reproductive plant tissues?

Both reproductive - seed - and non-reproductive - other - plant tissues have desiccation sensitive and tolerant types!

01 February 2015 8,164 2 View

Is the classification of scientists according to ResearchGate scores valid?

ResearchGate scores can be classified into: (1) < 10: beginner and/or poor, (2) 1- 20: mediocre and/or senior, (3) 20 - 30: good and/or senior, (4) 30 - 40: excellent and/or world-leading, (5) >...

05 June 2014 2,470 5 View

Is it true that all viral diseases are incurable?

Patrick Leburu: "Viral infections may be incurable! However, some may be treatable!"

05 June 2014 7,063 2 View

What are your views on 'emotional intelligence'?

Intelligence is indivisible!

03 April 2014 8,027 10 View

Is constant mutation the (sole) cause of the incurability of viral disease(s) and/or infection(s)?

Viruses constantly mutate!

31 December 2013 8,255 8 View

Is autophagy a combination and/or hybrid of (elements of) apoptosis and necrosis?

Apoptosis is programmed cell death! Necrosis is not!

09 October 2013 6,758 22 View

Is there (a) mechanism(s) for drug resistance other than its efflux?

Drug resistance is usually mediated by drug efflux.

06 July 2013 9,056 6 View

Does coinfection affect and/or influence host immunological responses to each infection?

Possibly and/or probably!

01 January 1970 5,145 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View