What are mediators of apoptosis?

More Tobias M. Ntuli's questions See All

What is the active ingredient in tobacco?

The active ingredients in alcohol and ganja and/or marijuana are ethanol and cannabis, respectively!

05 June 2016 5,131 3 View

Which is the better method between high-resolution scanning and/or freeze-fracture transmission electron microscopy for lipid phase-transitions study?

Used high-resolution scanning electron microscopy for the study of lipid phase-transitions in desiccation-responses of wild rice in conjunction with the standard freeze-fracture a decade ago

06 July 2015 6,287 10 View

What are the differences in desiccation sensitivity and tolerance mechanisms in reproductive and non-reproductive plant tissues?

Both reproductive - seed - and non-reproductive - other - plant tissues have desiccation sensitive and tolerant types!

01 February 2015 8,011 2 View

Is the classification of scientists according to ResearchGate scores valid?

ResearchGate scores can be classified into: (1) < 10: beginner and/or poor, (2) 1- 20: mediocre and/or senior, (3) 20 - 30: good and/or senior, (4) 30 - 40: excellent and/or world-leading, (5) >...

05 June 2014 2,328 5 View

Is it true that all viral diseases are incurable?

Patrick Leburu: "Viral infections may be incurable! However, some may be treatable!"

05 June 2014 6,842 2 View

What are your views on 'emotional intelligence'?

Intelligence is indivisible!

03 April 2014 7,868 10 View

Is constant mutation the (sole) cause of the incurability of viral disease(s) and/or infection(s)?

Viruses constantly mutate!

31 December 2013 8,097 8 View

Is autophagy a combination and/or hybrid of (elements of) apoptosis and necrosis?

Apoptosis is programmed cell death! Necrosis is not!

09 October 2013 6,616 22 View

Is there (a) mechanism(s) for drug resistance other than its efflux?

Drug resistance is usually mediated by drug efflux.

06 July 2013 8,888 6 View

Does coinfection affect and/or influence host immunological responses to each infection?

Possibly and/or probably!

01 January 1970 4,995 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View