In the HPLC, we can use differents detector methods to quantify the cannabinoid content from the flowers of Cannabis sativa. Most populars detectors are:
1. UV
2. Diodo Array Detector
3. Mass spectrophotometer
Which one you condider better and why?
Due to the large variety of cannabinoids, the use of MS detector is better than Uv and DAD detractors for their determination because the spectra overlap in the UV and DVD detectors is higher than MS detector.
The presence of MS detector database can also help identify these compounds easily.
Please look at the following below links which may help you in your analysis:
https://www.sielc.com/cannabis-analysis
https://jast-journal.springeropen.com/track/pdf/10.1186/s40543-018-0159-8
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma-Aldrich/General_Information/1/cannabis-testing.pdf
Thanks
When analyzing a range of compounds, the answer is the same for most applications. The best detector to use in this application is a scanning DAD coupled inline to a MS detector (MS or MS/MS). The reasons are practical ones. Many compounds do not ionize at all or well under common LC/MS analysis conditions so the MS may not detect critical compounds or other unknowns (an MS detector is not a universal detector). The same concern is valid for UV/VIS too. Depending on the extinction coefficient of the compounds chromaphore's (or the possibility that the compound does not have a strong enough or any chromaphore at all) some compounds may not be detected by UV/VIS. It is for this reason that we employ both detectors, inline, as standard for this type of analysis. Both detection systems complement one another. They provide various dimensions of orthogonal data which is a requirement in HPLC method development.
Another critical point overlooked by many is that you must in all cases follow good HPLC method development guidelines and FIRST develop a proper HPLC method which retains, resolves (not co-elute), baseline separates and then elutes all compounds under analysis. These basic requirements insure that the method used is selective for the compounds under analysis and each compound is baseline resolved. That provides the first dimension of analysis (Retention). Good methods are reproducible, rugged, practical and easy to run. Well resolved peaks on stable baselines allow the computer's software to accurate integrate and report the results. Adding UV/VIS spectra and peak ionization data (MS or MS/MS) add two or more dimensions of data.
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