Which is the best the detector in HPLC for test cananbinoids?

In the HPLC, we can use differents detector methods to quantify the cannabinoid content from the flowers of Cannabis sativa. Most populars detectors are: 1. UV 2. Diodo Array Detector 3. Mass...

19 September 2019 459 3 View

BD CBA cytokine kit assay use with BDFACSDiva and Flowjo?

Does anyone know if the CBA cytokine assay offered by BDbiosciences is compatible to run with BDFACSDiva software on a flow cytometer (vs the BDFACSSuite software that is recommended in the user...

15 January 2019 5,663 5 View

What is the best way to convert Multiband sequence DICOMs into Nifti?

Normally for functional images in the past I've used Xiangrui Li's Matlab script or mricron's dcm2nii but this is the first time I am working with Multiband data and have been running into...

12 April 2018 5,553 3 View

When performing multicolor flow cytometry can one use several different color panels in one experiment?

So let's say I have 3 samples and I am interested in testing different fluorophore-conjugated antibodies against certain antigens by using 12 different colors. Howeveer, aach sample only has 10...

05 April 2018 4,083 2 View

Why do I get fluctuating peak areas?

I carried out an experiment aimed at confirming the presence of aspirin and to quantify the abundance of asprin using standard addition. And using HPLC instrumentation. From the result, the...

05 April 2018 5,916 6 View

ELISA assays: Negative results for known positives?

I am testing insoluble homogenates diluted to 0.25M guanidine HCL. Standards for every kit tested work beautifully (standards also in guanidine HCL dilution. The samples are from human gene KI...

22 January 2018 8,822 2 View

Is there a formula in computing the retention factor values of lichen substances for the solvent dichloromethane?

I am currently working on the identification of lichen substances (secondary metabolites) of manglicolous lichens and I only used thin layer chromatography. The solvent I used is dichloromethane....

06 October 2017 7,939 1 View

When staining targets from 2 different species for flow cytometry, can you use 2 different primary Abs against different species in the same sample?

(if you staining for a mouse target and human target within one mouse (ex, chimera), can this be stained in the same tissue sample? Or should samples be stained separately?)

09 August 2017 7,149 2 View

Can anyone explain why I get a lower effect estimate using two-stage logistic analyses compared to straightforward logistic regression?

I am using a two-stage approach (whereby admissions to hospital are nested within patients) to account for multiple admissions of individual patients during a study period. Intuitively it makes...

21 September 2016 8,379 4 View

Are there available ID keys to marine epiphytes associated with seagrasses?

16 August 2016 395 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Does qualitative research using closed and open ended survey and semi structured interviews qualify as triangulation?

I am using qualitative research to look at employee motivation. I will use a closed and open ended survey (for biodata information and general views to motivation) and semi structured interviews...

18 February 2021 436 11 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Can anyone recommend a scale to measure Bloom's taxonomy of learning styles?

I am looking for recommendations of scales (in English or Spanish) to measure learning styles according to Bloom's taxonomy of learning. Thank you in advance!

09 February 2021 1,419 8 View

Can anyone suggest papers in the hybrid auto-mata area that models cyber-physical systems in power systems?

Most of the hybrid automata papers target formal verification of the systems. I am looking for papers that are solving optimal response problems that propose optimal control in power systems under...

07 February 2021 5,233 1 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View

Numerical taxonomy with SPSS?

Hi everyone, I was wondering about the possibilities of performing numerical taxonomy with SPSS software. I would be very thankful to recieve advice!! For now I have been reading about...

12 January 2021 5,549 3 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

How can we say as a plant native to a particular place?

Adorable experts, please enlighten me from this, on which basis we will say a plant native to a particular place. If is there any books or online webs suggest me. Thanks, advancially, With...

24 December 2020 918 14 View