Which is the best the detector in HPLC for test cananbinoids?

In the HPLC, we can use differents detector methods to quantify the cannabinoid content from the flowers of Cannabis sativa. Most populars detectors are: 1. UV 2. Diodo Array Detector 3. Mass...

19 September 2019 459 3 View

BD CBA cytokine kit assay use with BDFACSDiva and Flowjo?

Does anyone know if the CBA cytokine assay offered by BDbiosciences is compatible to run with BDFACSDiva software on a flow cytometer (vs the BDFACSSuite software that is recommended in the user...

15 January 2019 5,663 5 View

What is the best way to convert Multiband sequence DICOMs into Nifti?

Normally for functional images in the past I've used Xiangrui Li's Matlab script or mricron's dcm2nii but this is the first time I am working with Multiband data and have been running into...

12 April 2018 5,553 3 View

When performing multicolor flow cytometry can one use several different color panels in one experiment?

So let's say I have 3 samples and I am interested in testing different fluorophore-conjugated antibodies against certain antigens by using 12 different colors. Howeveer, aach sample only has 10...

05 April 2018 4,083 2 View

Why do I get fluctuating peak areas?

I carried out an experiment aimed at confirming the presence of aspirin and to quantify the abundance of asprin using standard addition. And using HPLC instrumentation. From the result, the...

05 April 2018 5,916 6 View

ELISA assays: Negative results for known positives?

I am testing insoluble homogenates diluted to 0.25M guanidine HCL. Standards for every kit tested work beautifully (standards also in guanidine HCL dilution. The samples are from human gene KI...

22 January 2018 8,822 2 View

Is there a formula in computing the retention factor values of lichen substances for the solvent dichloromethane?

I am currently working on the identification of lichen substances (secondary metabolites) of manglicolous lichens and I only used thin layer chromatography. The solvent I used is dichloromethane....

06 October 2017 7,939 1 View

When staining targets from 2 different species for flow cytometry, can you use 2 different primary Abs against different species in the same sample?

(if you staining for a mouse target and human target within one mouse (ex, chimera), can this be stained in the same tissue sample? Or should samples be stained separately?)

09 August 2017 7,149 2 View

Can anyone explain why I get a lower effect estimate using two-stage logistic analyses compared to straightforward logistic regression?

I am using a two-stage approach (whereby admissions to hospital are nested within patients) to account for multiple admissions of individual patients during a study period. Intuitively it makes...

21 September 2016 8,379 4 View

Are there available ID keys to marine epiphytes associated with seagrasses?

16 August 2016 395 1 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

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The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Defining effective diffusivity in porous zone and non-porous zone?

Hi all, I am working on methanol steam reforming using packed bed for hydrogen generation. I am using FLUENT software for CFD simulation. In reference paper, authors mentioned that effective mass...

10 February 2021 6,104 1 View