Good day all, I am about starting an experiment, in which I have to induce colon cancer.

(1). Please can anyone tell me the best model to use among these; Methylazoxymethanol (MAM), 1,2-dimethylhydrazine (DMH), and azoxymethane (AOM). And also, how long will each of these agent take to induce colon cancer in rats.

(2). Can 1,2-dimethylhydrazine and azoxymethane be combined?

(3). What is the best age to induce colon cancer in rats for both sexes?

I would be grateful if anyone can help.

Thanks in advance

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