Which is a preferred buffer for Laccase assay using guaiacol as a substrate?

More Lalita Limaye's questions See All

Can ethyl acetate extraction of buffered enzyme reaction prior to chiral column HPLC remove Tris salt & NADPH from the reaction sample selectively?

Hi! Using Chiral HPLC column (OB-H), I am trying to analyze final enzymatic reaction products of NADPH dependent aldo ketoreductase for possible formation of asymmetrical alcohols of...

20 August 2021 7,875 9 View

D/L or R/S chemicals, are these racemic? If I purchase such chemicals, can I obtain separate peaks for each orientation of these isomers?

Dear all, I am very new to chiral HPLC. Can someone help me understand a thing regarding chiral HPLC : I am studying stereoselective reduction of bulky ketones by recombinant aldo-keto...

15 April 2021 8,801 3 View

How to interpret values derived from Morphometric Analysis for a basin ?

1.Mean Bifurcation Ratio (Rb) 2.Drainage Density 3.Stream Frequency 4. Form Factor 5.Stream length Ratio (RL) I am able to find values for the above using certain formulae. But, how do I know...

07 April 2021 9,776 4 View

How to calculate MIC and MBC of some compound against bacteria?

Hello everyone, I am looking for the protocol to calculate MIC and MBC by broth microdilution method but I am unable to get a suitable protocol. In papers, they have given the reference of CLSI...

17 March 2021 5,286 7 View

What is the composition of TM buffer used for concentrating bacteriophage?

Hello everyone, I have to isolate DNA from Brucella phage , but before that, I have to concentrate the phage using PEG 8000. I have got protocol for concentrating the phage using PEG and TM buffer...

24 January 2021 3,065 6 View

How to interpret the result of agar well diffusion assay using herbal extract?

I performed the antibacterial activity of isolated herbal extract against Rhodococcus equi and used Erythromycin as a standard drug, ZOI of the plant extracts were 16-20mm and Erythromycin was 28...

17 January 2021 1,977 3 View

How to find out co factor specificity of aldo keto reductase enzymes?

I have expressed 3 recombinant Aldo keto reductase genes from Gram negative bacterium belonging to the genus Chryseobacterium. Will I have to purchase and try all the cofactors like NADP, NADH,...

23 November 2020 2,837 1 View

Has anyone done research on CAD modelling for 3 Wheelers for design optimization & weight Reduction?

We are working on the light weighting projects with customers manufacturing 3 Wheeler, 2 Wheeler & Small Commercial Vehicles. We would be glad to have someone whos has done research in 2 &...

05 May 2020 1,424 0 View

If I store β-NADPH stock solution prepared in Na-bicarbonate buffer, pH9 as per SIGMA, can I use this in reaction with pH other than 9 ?

I am planning to make a single time stock solution of β-NADPH (10mM, 100x concentration) from 100 mg desiccated powder and store its single-use aliquot vials at -20C refrigerator. I am a bit...

02 December 2019 5,103 0 View

Do all the mRNAs of pox virus are polyadenylated at 3' end?

Hello everyone, In eukaryotic cells, all the mRNAS are polyadenylated at 3' end. I want to ask whether this is the same case for pox viruses also. Do all their mRNAs are polyadenylated at 3'end...

03 May 2019 9,524 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View

How much 1X TAE buffer should I use in preparing 1.2% agarose gel?

Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...

12 July 2024 2,058 5 View

My Cyclic voltammetry curve doesn't show cathodic peak of Cu2+ to Cu+. how to solve this problem?

I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.

08 July 2024 2,388 4 View