How can I apply a thermo-mechanical load in hypermesh?

I want to determine the stresses for a laminated composite plate under thermo-mechanical load.

24 May 2019 9,180 4 View

Can I get MATLAB code for vibrational analysis of composite plate?

Iam working on vibrational analysis of laminated composite plate using MATLAB.

28 October 2018 7,641 3 View

Is there any effect of acids,bases on the activities and properties of polyphenols?

Do polyphenols behave differently on interaction with acids and bases?If yes what are the possible changes in the properties?

15 May 2018 1,543 3 View

How we can determine individual sum of squares for independent variables (factors) in the central composite design?

I am trying to solve central composite design ANOVA table manually. It has total 30 number of runs which includes 16 factorial runs,8 axial runs and 6 center runs. so, far using regression method...

23 April 2018 2,103 1 View

Why carcinoma cell lines (e.g. HepG2, Caco-2, RAW) are used in invitro cytotoxicity experiments?

HepG2 is considered as a model system for various cytotoxicity experiments according to many studies. Won't it produce wrong results if carcinoma cells have been used for such studies as such...

21 August 2017 5,310 7 View

What is the best place to find high power LEDs for GFP illumination?

I am looking for a high power LED with a central wavelength of around 488 nm. I know Thorlabs sells one at 490 nm that supplies 216 mW of optical power at 488 nm. However, I need at least 1 W at...

01 June 2017 4,234 3 View

Is there any standard criterion to select optimum number of sequence candidates for establishing a phylogeny tree in bacteria using MEGA?

for example, in case of 16s rRNA nBLAST results, I have almost 80 similar sequences with % similarity ranging from 99% and going down till 94% ?

16 March 2017 4,772 2 View

Nusselt Number corelation for hot liquid flow through inner annulus of pipe in case of Laminar flow.

I encountered a problem in my project work on heat exchangers where to find heat transfer coefficients on the defined conditions the Reynolds number came to be 1753.8 while the Prandtl number is...

08 March 2017 4,652 3 View

Can a substitution mutation in an exon result in splice site changes?

I have found a novel mutation in clotting factor 2 gene in an exon (midway through the exon), however, on checking with mutation tester it is showing that the mutation may result in splice site...

01 March 2017 8,737 3 View

I am trying Laccase assay with ABTS using Mc Ilvaine's buffer.Is there any other buffer?The color of my substrate solution colorless? Is it correct ?

18 February 2016 3,972 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View