Lalita Limaye

6 Questions 8 Answers 0 Followers

Questions related from Lalita Limaye

I am trying Laccase assay with ABTS using Mc Ilvaine's buffer.Is there any other buffer?The color of my substrate solution colorless? Is it correct ?

How much is the OD change observed? Reading taken only once after one minute OR for 10 minutes after every minute ?

19 February 2016 4,181 2 View

Which is a preferred buffer for Laccase assay using guaiacol as a substrate?

I am trying to carry out assay at pH 7 using McIlvaine's buffer or phosphate buffer. Which buffer should be used?

11 August 2015 2,592 3 View

What is the difference between 2,4 xylidine and 2,5 xylidine as an anducer molecule?

2,4 xylidine can be used as an inducer for enzyme Laccase from actinomycetes. But in literature why is it indicated that 2,5 xylidine is a preferred inducer as compared to 2,4 xylidine ?

06 October 2014 1,213 3 View

DNSA method for the estimation of Xylanase activity. What is the right protocol to follow: Baileys or Millers?

I take the std xylose in the range 100-1000ug/ml. Protocol I follow is 1mlstd.+1mlDNSA. Boil and add 8ml water. For unknown I take 1ml metabolic filtrate+1ml substrate+1ml DNSA.Boil and add 7ml...

15 August 2014 1,233 7 View

What is the right source of sample for getting Actinomycete which is Laccase positive ?

I am trying to identify actinomycete which is a laccase producer. I hv tried 27 isolates but none gave positive results on SCN Medium with Guaiacol.

13 August 2014 7,485 5 View

Which medium should be used for plate assay to detect Actinomycete producing Laccase?

Plate assay for detection of Laccase producer.

02 August 2014 9,555 3 View