How can I apply a thermo-mechanical load in hypermesh?

I want to determine the stresses for a laminated composite plate under thermo-mechanical load.

24 May 2019 9,180 4 View

Can I get MATLAB code for vibrational analysis of composite plate?

Iam working on vibrational analysis of laminated composite plate using MATLAB.

28 October 2018 7,641 3 View

Is there any effect of acids,bases on the activities and properties of polyphenols?

Do polyphenols behave differently on interaction with acids and bases?If yes what are the possible changes in the properties?

15 May 2018 1,543 3 View

How we can determine individual sum of squares for independent variables (factors) in the central composite design?

I am trying to solve central composite design ANOVA table manually. It has total 30 number of runs which includes 16 factorial runs,8 axial runs and 6 center runs. so, far using regression method...

23 April 2018 2,103 1 View

Why carcinoma cell lines (e.g. HepG2, Caco-2, RAW) are used in invitro cytotoxicity experiments?

HepG2 is considered as a model system for various cytotoxicity experiments according to many studies. Won't it produce wrong results if carcinoma cells have been used for such studies as such...

21 August 2017 5,310 7 View

What is the best place to find high power LEDs for GFP illumination?

I am looking for a high power LED with a central wavelength of around 488 nm. I know Thorlabs sells one at 490 nm that supplies 216 mW of optical power at 488 nm. However, I need at least 1 W at...

01 June 2017 4,234 3 View

Is there any standard criterion to select optimum number of sequence candidates for establishing a phylogeny tree in bacteria using MEGA?

for example, in case of 16s rRNA nBLAST results, I have almost 80 similar sequences with % similarity ranging from 99% and going down till 94% ?

16 March 2017 4,772 2 View

Nusselt Number corelation for hot liquid flow through inner annulus of pipe in case of Laminar flow.

I encountered a problem in my project work on heat exchangers where to find heat transfer coefficients on the defined conditions the Reynolds number came to be 1753.8 while the Prandtl number is...

08 March 2017 4,652 3 View

Can a substitution mutation in an exon result in splice site changes?

I have found a novel mutation in clotting factor 2 gene in an exon (midway through the exon), however, on checking with mutation tester it is showing that the mutation may result in splice site...

01 March 2017 8,737 3 View

I am trying Laccase assay with ABTS using Mc Ilvaine's buffer.Is there any other buffer?The color of my substrate solution colorless? Is it correct ?

18 February 2016 3,972 2 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

QTL, recombination frection?

22 February 2021 2,588 2 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Where to buy optically clear, low viscosity, refractive index 1.5-1.4 Silicone Oil ?

For research purposes, it is required to have optically clear, low viscosity silicone oil with a refractive index of 1.5-1.4. It is going to be used in optoelectronic research. Online purchase is...

01 February 2021 9,160 3 View

Recombinant restriction enzyme: how strong should the corresponding methyltransferase expression level be?

Hello. We are planning expression of a restriction enzyme in E. coli, and wondering how much of the corresponding methyltransferase is needed to be expressed in order to protect the host genome...

23 January 2021 2,096 4 View