I performed the antibacterial activity of isolated herbal extract against Rhodococcus equi and used Erythromycin as a standard drug, ZOI of the plant extracts were 16-20mm and Erythromycin was 28 mm, are these herbal extracts are effective or not.
What is the criteria to consider any agent to consider as good/bad antibacterial.
You can't make a direct comparison to erythromycin because (1) you don't know the concentration of the active substance(s) in the herbal extract; and (2) the rate of diffusion of an active substance depends on the properties of the individual substance, such as its molecular weight, concentration, and interaction with the agar. All you can say is that the extract showed antibacterial activity. You could use this as a quantitative way of comparing the potencies of different preparations of the same extract. The erythromycin just served as a positive control.
Here is a paper that you can use to find your answer:
Article Methods for in vitro evaluating antimicrobial activity: A review
You can see my article
https://www.hindawi.com/journals/jfq/2020/8861395/
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