Hi,
Which growth factor accelerates the division of lung cancer cells? I need an answer specific to lung cancer cells not a general answer.
Thanks.
0ver-expression of the phosphorylated EGFR protein accelerates the division of lung cancer cells.
Joe Graymer
I work mainly on NSCLC typically on A549 cell line. Is there any growth factor that speed up the growth of this cell line?
Epithelial cells should theoretically respond to EGF, but practically cells in labs seem to reject theories. If EGF isn't working, perhaps FGF7 could help. I'd read a paper about its role in A549 somewhere.
Iman Hassan Ibrahim thank you so much! However, I would like to ask you whether you have already tested FGF7 on A549 or any other lung cancer cell line. I already tried EGF but as you said cells are practically non responsive.
No I didn't test it, I read a paper reporting the use of FGF7 on A549. I'll try to get the link for you.
EGF promotes growth, proliferation and colonizing ability of the A549 lung adenocarcinoma cell line. Through upregulation of claudin-2, EGF can enhance colonization of A549 cells, as claudin-2 loosens the tight junctions between cells. IGF-I acts as a proliferation and growth inhibitor for the A549 cell line.
Hamadi Madhi
Then why so many articles state that EGF is stimulatory to the growth of A549 cells...???
Peter Y, Comellas A, Levantini E, Ingenito EP, Shapiro SD. Epidermal growth factor receptor and claudin-2 participate in A549 permeability and remodeling: implications for non-small cell lung cancer tumor colonization. Mol Carcinog. 2009 Jun;48(6):488-97. doi: 10.1002/mc.20485.
Croxtail JD et al. Lipocortin 1 and the control of cPLA2 activity in A549 cells: Glucocorticoids block EGF stimulation of cPLA2 phosphorylation. Biochemical Pharmacology, 52(2): 1996: 351-6.
Ono M, Hirata A, Kometani T, Miyagawa M, Ueda S, Kinoshita H, Fujii T, Kuwano M. Sensitivity to gefitinib (Iressa, ZD1839) in non-small cell lung cancer cell lines correlates with dependence on the epidermal growth factor (EGF) receptor/extracellular signal-regulated kinase 1/2 and EGF receptor/Akt pathway for proliferation. Mol Cancer Ther. 2004 Apr;3(4):465-72.
Jaramillo M, Paul-Roc B, Grothe S, Banville M, O’Connor M. Effect of blocking EGF receptor function in A549 lung carcinoma cell lines using a neutralizing antibody on cell signaling, growth, motility and epithelial-to-mesenchymal transdifferentiation. Proc Amer Assoc Cancer Res, Volume 45, 2004.
Zhou P et al. Epidermal growth factor receptor expression affects proliferation and apoptosis in non-small cell lung cancer cells via the extracellular signal‑regulated kinase/microRNA 200a signaling pathway. ONCOLOGY LETTERS 15: 5201-5207, 2018.
Nishimura Y et al. EGF‑stimulated AKT activation is mediated by EGFR recycling via an early endocytic pathway in a gefitinib‑resistant human lung cancer cell line. Int J Oncol 46(4): 1721-1729, 2015. doi Article EGF‑stimulated AKT activation is mediated by EGFR recycling ...
Jaramillo ML et al. Differential sensitivity of A549 non small lung carcinoma cell responses to epidermal growth factor receptor pathway inhibitors. Cancer Biol & Ther, 7:4, 557-68, 2008. DOI: 10.4161/cbt.7.4.5533.
I hope that these help.
I would propose to perform a literature search using adequate sources (e.g. PubMed).
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