I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted microscope. How can I calculate the % of viability without staining the cells or doing MTT ?
i would appreciate any help
You could add a live/dead staining (ie. Trypan blue) to the cells and then take images to analyze the cells survival.
I am unsure of what kind of phenotype you would get once you added the bacterial filtrates to the cells. Do the cells get lysed or get round-up? I have seen publications that measure the cell viability by counting the % of cell rounding based on microscopy images.
alternatively, XTT assay would be a good substitute for MTT assay. They work in similar ways, but Xtt assay is more straightforward in procedures.
Thank you for replying Mr.Jianfeng Lin ,
Adding live/dead staining is a good idea , but I already have the images after 24 h incubation that’s why I was looking for a method to do the counting without any staining . After incubating cancer cells with bacterial filtrates their morphology changes to get round-up and circular .
XTT is a good suggestion though would you mind recommending some publications related to this assay ?