Which fluorescent tag is better for my 120 kDa protein?

30 July 2015 1 8K Report

Hello Everyone.. I want to label my 120kDa protein with a fluorescent tag without altering any of its properties. We have GFP, FITC and Alexa Fluor in our lab..which one is better?

Marcin Nowicki

Are you limited to these tags? If not, I would recommend the FLaSH tag :shorter, less problematic, and with potential to later isolate your protein by binding. Also: http://onlinelibrary.wiley.com/doi/10.1002/jbio.201100018/pdf

FlaSH: http://www.lifetechnologies.com/pl/en/home/life-science/cell-analysis/cellular-imaging/high-content-screening/flash-and-reash.html

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