Hi Thounaojam

you can use the neutral detergent fiber and acid detergent fiber to determine cellulose, hemicellulose and lignin.

You can follow the NREL standard protocol to determine the biomass composition (lignin, cellulose, and hemicellulose)

http://www.nrel.gov/biomass/analytical_procedures.html

Hi,

Determination of lignin, cellulose and hemicellulose content in rice straw can be performed by using standard methods applicable to wood or any other lignocellulosic materials.  I will attach summary for some standard methods that you can use for analysis of rice straw:

Hi

you can follow the acetyl bromide method

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0110000

this is a good and simple method.

I recommend Mood's answer

Best Regards

I hope that this methodology could help you.