1st you have to prepare media which can be Malt extract broth or can be general as potato dextrose broth and incubate fungi for 14 days approx., if you want cell free suspension then centrifuge the media and further filter it if required (preferable and authentic). However if you do need some cells in that then just separate the mycelium by septic process in laminar. You can consult my paper (attached) for same.
Article Screening of potential biotechnological applications from ob...
For the spore suspension , the use of Tween 80 at 1 percent solution would be helpful.
Grow the mold on Potato Dextrose agar for~ 7 days at 35C then cover the colonies with1 ml 0.85% Saline containing 1 drop (0.01 ml) of Tween 20. Withdrawn the mixture to a sterile tube allowed 5 minutes for the heavy particles to settle down and transfer the upper homogenous suspension to a sterile tube you may filter it through gaze or gently centrifuge it.
use slant contain PDA and activated Aspergillus for 7 day and add pepton water 5ml to culture then take 1 ml from suspension and counted .
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