Which density gradient medium to use for PBMC isolation?

15 February 2013 0 9K Report

Are there any reasons for choosing any particular brand of density gradient medium over another for PBMC isolation, other than cost? ie. Ficoll-Paque PLUS or PREMIUM, Lymphoprep or Histopaque-1077?

Badges
Science topic

Similar topics
Medicine
Epi
Age Groups
Children

More Aedan Roberts's questions See All

Where can I acquire the Dimension of Administrative Support Inventory Scale?

Needed for a dissertation,

17 July 2024 2,004 0 View

Overheating with western blot transfer - likelihood of protein transfer?

Hello, I've just attempted a wet transfer of a western, and had set up the cassette and tank, and run @ 100V and set it to 90 mins. I had pre-chilled the transfer buffer - but didn't put the...

25 June 2024 7,419 2 View

How to write a proposal for resolving workplace conflicts?

I am writing a research paper on resolving workplace conflict. I need help writing problem statement and proposal and any suggestions for references.

19 March 2024 8,986 3 View

What is the most recent thought on bison as part of the historical fauna in the U.S. desert southwest?

I have been in the camp that believed bison were not part of the natural fauna in the desert grasslands. Individual bones found scattered throughout were as a result of trade among indigenous...

09 December 2023 2,452 1 View

How do I correct the title of an artilce that is already uploaded in Research Gate?

Now lists on a partial title.

02 December 2023 9,661 1 View

Purification of StreptagII for CoIP?

I have been attempting to purify a streptagII-tagged protein from mammalian cells using streptavidin-magnetic beads without success. The reason I'm using streptavidin and not streptactin is that...

08 March 2023 367 0 View

How to do I work out the volume of my adiabatic packed bed reactor when synthesising ammonia?

I’m Using a water gas shift reaction with a high temperature shift in an adiabatic Packed bed reactor to synthesise ammonia. I’m struggling to find the correct rate equation and therefore volume...

28 February 2022 3,883 3 View

What 2-3 evidenced-based social skills and executive function interventions are recommended in children and adolescents with FASD?

Hi For an assignment I need to critically evaluate social skills interventions and executive functions that are recommended specifically for children between 6 - 12 with FASD but I'm struggling to...

20 February 2022 2,773 1 View

Does anybody know where I could find the constructivist learning environment survey?

I am doing a project for the final module of my degree with the OU, and looking at the relationship between epistemological beliefs and peoples attitudes towards constructivist online learning...

09 January 2022 5,485 4 View

How do I statistically analyze motility of c. elegans?

Working on a research project and we have performed a motility assay on c. elegans with different essential oils. Wondering if there was any way to test if it statistically significant.

28 November 2021 6,383 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View