Parsing error in autodock vina?

I'm trying to use autodock vina, and I received the following error for the ligand file when running the configuration: "Parse error on line 26 in file "15.pdbqt": Unknown or inappropriate...

21 October 2020 7,245 1 View

Does anybody have any knowledge of "dry" microbiological decontamination methods for frozen fruit?

I am looking for data on the implementation of a microbiological reduction step for frozen fruit pieces. I have information on cold plasma/UV but am unable to locate much data on gaseous methods...

24 November 2019 6,131 8 View

Has anyone used the MinuteTM lysosome isolation kit to isolate lysosomes?

This seems to be the only kit (made by Invent Biotechnologies) that's based on a non-density gradient and ultracentrifugation method. Has anyone used it successfully to isolate lysomes from...

20 November 2019 7,210 3 View

Has anyone used the MinuteTM lysosome isolation kit to isolate lysosomes?

This seems to be the only kit (made by Invent Biotechnologies) that's based on a non-density gradient and ultracentrifugation method. Has anyone used it successfully to isolate lysomes from...

10 November 2019 9,907 3 View

Can tissues dissolve in formalin?

My question pertains to human heart tissue previously embedded and frozen in OCT. We have recently cut sections of varying thickness (100-1000 um) for processing and staining while free-floating....

21 October 2019 7,148 3 View

Do I treat Likert scale data (predictors) with continuous DV as parametric or non-parametric?

Hi there, I am carrying out a study on factors affecting electric vehicle adoption. I am using a likert scale format (from strongly disagree to strongly agree) for all of the predictor variable...

13 August 2019 258 3 View

Do I use multiple regression or hierarchy multiple regression?

My study is on electric vehicles and I want to test whether factors such as cost and range anxiety have significant effects and their degree of influence when analysing the intent to adopt. I am...

03 July 2019 9,547 10 View

Are there any drawbacks of using Peak Area instead of Peak Height to calculate ratio of alleles in fragment analysis?

I am setting up a fragment analysis assay in which I need to calculate the ratio of the two peaks. The protocol I have inherited suggests using Peak Height, and I see this used a lot generally for...

07 May 2019 9,369 1 View

RNA Free Energy?

If you have a validated RNA structure (with or without pseudoknots) can you back calculate a theoretical free energy for that structure? I know many programs that calculate a free energy and try...

20 January 2019 7,763 2 View

Are there any Excel lookup functions that return more than one value?

Hi, I'm looking to incorporate a formula into a database I'm building in Excel that returns the identification of a chemical based on it's linear retention index (LRI). Some of these chemicals...

26 April 2018 1,779 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View