Aedan Roberts

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Questions related from Aedan Roberts

Diluting blood in PBS or RPMI for PBMC isolation from whole blood?

I'm trying to set up a protocol for PBMC isolation from whole blood using density gradient centrifugation. In a previous lab we diluted the blood in RPMI prior to layering, and washed the PBMCs in...

15 February 2013 5,454 21 View

Which density gradient medium to use for PBMC isolation?

Are there any reasons for choosing any particular brand of density gradient medium over another for PBMC isolation, other than cost? ie. Ficoll-Paque PLUS or PREMIUM, Lymphoprep or Histopaque-1077?

15 February 2013 9,109 0 View