Which concentration of 100% ethanol doesn't affect cell lines? As 0.1-0.5% DMSO is not affecting any cell line.

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Need suggestion for stable cell line generation?

Hello All! I am trying to generate HCT 116 stable cell line. I studied many protocols and proceeded as follow: 1) I cultured cells in 6 wells plates at density of 5 x 10^5. 2) At 70% confluency,...

10 November 2016 9,949 4 View

PRPF4B (NM_003913) Human cDNA Clone?

Hello, I am going to generate a mammalian stable cell line, over expressed with PRPF4B. For this purpose I am looking for PRPF4B (NM_003913) Human cDNA Clone, in Neomycin/Hygromycin resistant...

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How to efficiently collect macrophages from mouse blood?

I am trying to collect macrophages from the mouse blood, but till now I am unable to do this perfectly. I feel following problems and need some recommendations: 1) Blood in mouse is very less,...

10 November 2015 9,653 2 View

How can I get the data from this western blot picture?

Hello everyone, As you can see in the picture, I have run two samples on SDS-Page, and signals have been detected by PARP antibody. Control has no band, while the treated sample is giving band,...

05 June 2015 9,260 8 View

Has anyone tried stable transfection of HCT-15 cells?

I am in search of recommendations about stable transfection of HCT-15 cells regarding selection of vector and antibiotic. It's my first time with this experiment and and I am searching a suitable...

05 June 2015 2,978 2 View

Why am I always getting dark background in western blot?

Please look at the attached photo. I have tried to develop MMP-3 band in HL-60 and HCT-15 cells. Usually i get the dark black backgroung, although i do the washing for about 1 h with 5 and 10...

04 May 2015 5,982 9 View

How can one isolate cell membrane proteins from mammalian cells?

I would like to isolate cell membrane proteins from human colorectal cancer cells (HCT-15) and human leukemia cells (HL-60). I am in search of a suitable protocol. I have to order all the...

02 March 2015 7,709 8 View

Is Decursin Angelate available commercailly?

Hello All, I want to work with Decursin angelate, but i am not able to find it commercially by some company. Can any one tell me about its commercial availability?

02 March 2015 8,729 0 View

Does anyone have sample for Digital Data of Cell Cycle Histogram?

We know that flow cytometer giving us a histogram of cell cycle, uses some data from individual cells. Often these are FCS files. I mean that flow cytometer records the fluorescence of individual...

01 February 2015 7,651 2 View

Did someone use "Prostaglandin E2 Express EIA KIT"?

I am going to check the PGE2 secretion by using Prostaglandin E2 Express EIA KIT, cayman chemical. I am using it for the first time, so little hesitated. I have four samples, as below: (1) Control...

01 February 2015 8,282 2 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

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08 August 2024 8,329 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

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06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

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05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

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05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Danilo Zangani

EtOH is safe to use at 0.01%, DMSO will interact with a lot of cell functions at any concentration, so it should not be absolutely used

Salman Ul Islam

Thank you very much sir.

Surajit Dey

Ethanol is usually safe to use considering you will only add a small amount (with drug dissolved) in it. DMSO makes the cell more permeable to leakage and should be avoided if possible